[B2-Lys]-胰岛素的制备与性质  

Preparation and Properties of [B2-Lys] Insulin

作　　者：徐明华[1] 叶莺[1] 张新堂[1] 朱尚权[1] 

机构地区：[1]中国科学院上海生物化学研究所,上海200031

出　　处：《生物化学杂志》1992年第4期410-413,共4页

摘　　要：本文报道了用化学半合成途径从天然猪胰岛素制备[B2-Lys]-胰岛素的过程。人胎盘细胞膜胰岛素受体结合试验表明:[B2-Lys]-胰岛素的受体结合能力只有天然胰岛素的80％,降兔血糖作用与时间关系的结果表明它没有长效作用。本文还对这些结果进行了讨论。The semisynthetic method for preparation of [B2-Lys]-insulin from native porcine insulin has been described in this paper.The insulin receptor binding assay on human placental membrane showed that the receptor binding Capacity of [B2-Lys]-insulin was only 80% that of the native insulin, which is quite different from the increased insulin receptor binding capacity of [B3-Lys]-insulin.It was observed that the replacement of B2-Val by Lys in insulin molecule did not prolong its effect on lowering blood glucose in rabbit.The effect of N-terminal sequence of insulin B-chain on receptor binding has been discussed.

关 键 词：胰岛素 制备 

分 类 号：Q578.03[生物学—生物化学]