用Digoxigenin标记DNA探针作基因转移山羊的整合检测  

Integrated Dection of Gene Transfer Goats dy DNA Probe Labeled with Digoxigenin

作　　者：成国祥[1] 王捍东[1] 成勇[1] 吴维芬[1] 穆宗尧 李国忠[1] 

机构地区：[1]江苏农学院畜牧兽医系

出　　处：《生物技术》1992年第4期11-13,20,共4页Biotechnology

基　　金：江苏省科委自然科学基金资助项目

摘　　要：对采用外源基因原核显微注射法生产的基因转移山羊,用一种新的DNA非同位素标记法即地高辛配基标记DNA探针作外源基因整合检测.结果表明:地高辛标记核酸探针能检测出基因转移动物基因组中整合的外源基因.Gene transfer goats produced by the method of microinjection foreign gene into pronuclear were made integrated detection with a novel nonradioactive DNA labeling method, namely DNA probe labeling with Digoxigenin. The experiment result show that the foreign gere integrated in gene transfer animal's genomic DNA can be detected.

关 键 词：地高辛配基 转基因 整合检测 山羊 

分 类 号：S188[农业科学—农业基础科学]