菌紫质光循环中间体的时间分辨谱  

TIME-RESOLVED SPECTRUM OF PHOTOCYCLE INTERMEDIATE OF BACTERIORHODOPSIN

作　　者：胡坤生[1] 王敖金[1] 孙英[1] 

机构地区：[1]中国科学院生物物理研究所,北京100101

出　　处：《生物物理学报》1992年第3期397-400,共4页Acta Biophysica Sinica

基　　金：科学院院长基金资助

摘　　要：利用毫微秒时间分辨的吸收变化和微秒的闪光动力学光谱研究了室温下菌紫质光循环中间体的形成过程及衰减过程。实验结果表明,室温下,K_(610)和M_(412)中间体同样出现,L_(550)中间体还不能得到证实,有待于进一步研究。The fromation rate and the decay rate of photocycle intermediate of bac-teriorhodopsin were investigated at room temperature by ns time-resolved absorption spectrum and μs flash kinetic spectrum. It was shown that at room temperature, K610 and M412 were formed in photocycle, The identif iration of L550 in photocycle, remains to be further investigated.

关 键 词：菌紫质 光循环中间体 时间分辨谱 

分 类 号：Q734[生物学—分子生物学]