人中性粒细胞与淋巴细胞化学发光反应的比较  

COMPARISON OF CHEMILUMINESCENCE RESPONSE BETWEEN POLYMORPHONUCLEAR LEUBOCYTE AND LYMPHOCYTE IN HUMAN BLOOD

作　　者：张学军[1] 陈宜芳[1] 毕克菊[1] 刘亦芬[1] 

机构地区：[1]安徽医科大学微生物学教研室,合肥230032

出　　处：《生物物理学报》1992年第3期487-491,共5页Acta Biophysica Sinica

基　　金：安徽省教委资助课题

摘　　要：研究人中性粒细胞化学发光(PMNCL)反应与淋巴细胞化学发光(LY—CL)反应的差别。结果表明,刀豆蛋白A、植物血凝素,葡萄球菌蛋白A和(?)理酵母多糖刺激后,PMN—CL纯峰值高于LY—CL 3—10倍,而(?)抗(?)IgG刺激后,LY—CL的峰值则稍高于OMN—CL。氧自由基清除剂作用于PMN—CL和LY—CL反应的结果表明,L—组氨酸均明显抑制PMN—CL和LY—CL反应;苯甲酸钠和甘露醇抑制LY—CL反应的抑制率(IR)明显高于PMN—CL;而超氧化物歧化酶和过氧化氢酶抑制LY—CL的IR明显低于PMN—CL。结果提示,PMN—CL和LY—CL反应在CL形成的分子机制上并不完全相同。A comparative study between lymphocyte chemiluminesrence (Ly-CL) response and polymorphonudear leukocyte che miluminesrence (PMN-CL) response in kuman blood was investigated. Results showed that the net peaks of PMN-CL stimulated by concanavalin A (Con A), phytohaemagglutinin (PHA), staphy-lococcus protein A (SPA) and opsonized zymosan (OZ) were 3-10 times higher than those of Ly-CL, but the net peak of Ly-CL stimulated by sheep anti-human IgG was higher than that of PMN-CL. The effect of oxygen free radicals (ORs) Scavergers on PMN-CL and Ly-CL indicated that L-histidine inhibited obviously both PMN-CL and Ly-CL. However, inhibiting rates (IR) of Ly-CL by sodium benzoate and mannitol were higher than IR of PMN-CL and IR of Ly-CL by supcroxide dis mutase (SOD) and catalase were lower than IR of PMN-CL. Those data suggest that the molecular mechanism of PMN-CL response may not be similar to that of Ly-CL response.

关 键 词：中性粒细胞 淋巴细胞 化学发光 

分 类 号：R329.27[医药卫生—人体解剖和组织胚胎学]