不同状态血红蛋白对红细胞膜功能的影响  

THE EFFECT OF DIFFERENT STATE HEMOGLOBIN ON FUNCTION OF ERYTHROCYTE MEMBRANES

作　　者：许红[1] 张志鸿[1] 

机构地区：[1]复旦大学生理学和生物物理学系,上海200433

出　　处：《生物物理学报》1992年第3期481-486,共6页Acta Biophysica Sinica

基　　金：国家自然科学基金

摘　　要：用荧光猝灭法测定了不同状态游离血红蛋白和红细胞血影膜间的相互作用,观察到和氧合血红蛋白相比,脱氧血红蛋白和膜上高亲和位点的作用加强,而高铁血红蛋白和低亲和位点的作用加强。本文用低渗溶血速率、阴离子通透性、变形性等三个参量测定了这种相互作用的变化对红细胞膜功能的影响,并讨论了脱氧状态下这种改变的生理意义。It is well known that there are high and low affinity binding sites of Hb to erythroryte membrane. By means of fluorescent quenching of free Hb to both 12-AS labeled non-sealed and re-sealed ghost suspensions, we drew the conclusion that DeoxyHb mainly increased binding with high affinity site while MetHb mainly increased binding with the low affinity site, as compared to HbO2.Three parameters were used to characterize the effect of different types of Hb on membrane function (HbO2 as a control).(1) The rate of osmotic he-molysis. We found DeoxyHb decreased K2 (rate constant of the slow process) significantly while MetHb deereased both K1(rate constant of the fast process) and K2, which is in good agreement with the results obtained by fluorescent quenching. (2) Def or inability. We found both DeoxyHb aod MetHb increased Ci (the initial concentration of PEG for distinct formation of Rouleau), which means the less defor mability of erythrocyte. (3) Anion transport property. We found DeoxyHb decreased Ts (the permeability time of NO_2^(-)), which means that deoxygenation of erythroeyte can increase anion transport.Finally, the physiological importance of the effect of deoxygenation on me mbrane function was discussed.

关 键 词：血红蛋白 红细胞膜 渗透溶血速率 

分 类 号：R329.27[医药卫生—人体解剖和组织胚胎学]