N-甲基小檗胺对人心房肌细胞瞬时外向钾电流和延迟整流钾电流的作用(英文)  

Effects of N-methyl berbamine on transient outward potassium cu rrent and delayed rectifier potassium current in human atrial myocytes

作　　者：韩冬云[1] 李金鸣[1] 杨雪鹰[2] 聂宏光[1] 宋志国[1] 韩松[1] 

机构地区：[1]中国医科大学药理学教研室,辽宁沈阳110001 [2]中国医科大学第一临床学院心脏外科,辽宁沈阳110001

出　　处：《中国药理学与毒理学杂志》2003年第5期350-353,共4页Chinese Journal of Pharmacology and Toxicology

摘　　要：目的　动物实验表明N 甲基小檗胺 (NMB)通过抑制豚鼠心室肌细胞ATP敏感性钾电流和钙电流来发挥抗心律失常和抗心肌缺血作用 ,故进一步研究NMB对人心肌细胞电流的作用。方法　膜片钳制技术全细胞记录模式研究NMB对人心房肌细胞瞬时外向钾电流 (Ito)和延迟整流钾电流 (IK)的作用。结果　指令电位为 +60mV时 ,NMB 0 .1 ,1 ,1 0 μmol·L-1 分别使Ito幅值下降 (1 6± 4) % ,(2 5±4) %和 (49± 3) % ,使IK 幅值下降 (42± 6) % ,(47±7) %和(65± 3) %。结论　NMB对人心房肌细胞Ito和IK 均有抑制作用。AIM The previous studies on animals showed that N -methyl berbamine(NMB) could inhibit ATP sensitive potassium current and Ca 2+ current in ventricular myocytes of guinea pig and play an effective role in arrhythmia and myocardial ischemia. To further elucidate the ionic mechanism of NMB on human heart myocytes, the effects of NMB on transient outward potassium current (I to ) and delayed rectifier potassium current (I K) in human atrial myocytes were investigated. METHODS T he standard whole cell recording method was used. RESULTS At potential of +60 mV, NMB 0.1, 1, 10 μmol·L -1 decreased I to by (16±4)%, (25±4)% and (49±3)%, respectively, and decreased I K by (42±6)%, (47±7)% and (65±3)% (n=5 cells from 4 patients, P <0.05 ), respectively. CONCLUSION NMB depresses I to and I K in human atrial myocytes.

关 键 词：N-甲基小檗胺 膜片钳技术 心房 心肌细胞 钾通道 瞬时外向钾电流 延迟整流钾电流 

分 类 号：R96[医药卫生—药理学]