山莨菪碱对乙酰胆碱诱导的猫离体动脉内皮依赖性舒张反应的影响(英文)  

Effect of anisodamine on acetylcholine-induced endothelium-dependent vasorelaxation in cat isolated arteries

作　　者：王莉莉[1] 路新强[1] 汪海[1] 肖文彬[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《中国药理学与毒理学杂志》2003年第5期366-369,共4页Chinese Journal of Pharmacology and Toxicology

基　　金：国家 973计划项目(G19980 51112 )~~

摘　　要：目的　观察山莨菪碱对乙酰胆碱 (ACh)诱导的内皮依赖性血管舒张反应的影响。方法　采用猫离体血管功能实验 ,观察山莨菪碱对ACh诱发的内皮依赖性血管舒张反应的影响。结果　在猫肠系膜动脉、肾动脉和股动脉 ,山莨菪碱 0 .0 1～ 1 .0nmol·L-1 能够浓度依赖地抑制ACh诱导的内皮依赖的血管舒张反应。山莨菪碱抑制 1 0 μmol·L-1 ACh所诱导血管舒张的IC50 分别为 0 .2 36 ,0 .72 9和 0 .50 8nmol·L-1 ,山莨菪碱的拮抗作用符合非竞争性拮抗模式。此外 ,1 0nmol·L-1 山莨菪碱能有效拮抗ACh诱导的冠状动脉内皮依赖性舒张反应。结论　山莨菪碱能强效拮抗ACh诱发的内皮依赖性血管舒张反应 ,这种效应具有组织特异性的特点。AIM To examine the effect of anisodamine o n acetylcholine(ACh)-induced endothelium-dependent vasorelaxation. METHODS Using isometric-tension test, in isolated artery rings derived from cat femoral, renal, mesentary and coronary arteries, the effects of anisodamine on endothelium-dependent vasorelaxation induced by ACh were observed. RESULT S The endothelium-dependent vasorelaxation induced by ACh could be block ed by anisodamine in a concentration-dependent manner in the isolated preparati ons derived from cat mesentery, femoral and renal arteries, the value of IC 5 0 were 0.236, 0.729 and 0.508 nmol·L -1 , respectively, against ACh 1 0 μmol·L -1 . The antagonism of anisodamine was fit for non-competitive m ode. Moreover, anisodamine at concentration of 10 nmol·L -1 inhibited the endothelium-dependent coronary artery relaxation induced by ACh(P<0 .05). CONCLUSION Anisodamine has potent effect against ACh-induc ed endothelium -dependent vasorelaxation with the tissue specific property.

关 键 词：山莨菪碱 乙酰胆碱 内皮 依赖性血管 舒张反应 离体动脉 血管舒张 

分 类 号：R96[医药卫生—药理学]