Modulatory Effect of Pollen Extract on Apoptosis of Lymphocytes in the Elderly  

中药成分PE对老年人淋巴细胞凋亡的调控作用(英文)

作　　者：李玉梅 张春玲[2] 范芸[2] 刘俊达[2] 

机构地区：[1]广州老人院,广州510550 [2]卫生部北京医院老年医学研究所,100730

出　　处：《Journal of Chinese Pharmaceutical Sciences》2003年第3期154-159,共6页中国药学（英文版）

基　　金：SuppordedbytheNationalNaturalScienceofChina ,No .3 9770 860

摘　　要：Aim In this study, we investigated the changes of lymphocyte subpopulationand apoptosis process of lymphocytes in the elderly, and the modulatory effect of pollen extract(PE) on apoptosis. Methods Lymphocyte phenotypes were detected using indirect immunofluorescencetechnique. The proliferation responses were determined by MTT method. Flow cytometry and automaticimage analysis were performed to evaluate the apoptosis of lymphocytes. Results The proliferationresponses of lymphocytes in the elderly were lower than that in young adults. Decreased CD_(45) RA^+cells and increased CD_(45) RO^+ cells were found in lymphocyte population of aged people, comparedwith that of young adults. The CD_(45) RO^+ cells were prone to apoptosis. There is an inhibitoryeffect of PE on apoptosis of lymphocytes in the elderly. Conclusion It is implied that thesusceptibility of lymphocyte in the elderly to apoptosis depends on activation, so called,activation-induced cell death. Present results suggest that apoptosis of lymphocytes in aged peopleplay an important role in the pathogenesis of immunosenescence. Thus, a possibility is open fordevelopment of apoptosis-modulating drugs from pollen.目的　研究老年人淋巴细胞亚群及其细胞凋亡过程 ,探讨中药成分PE对细胞凋亡的调控作用。方法　用间接免疫荧光技术检测淋巴细胞表型 ,用MTT法测定淋巴细胞增殖应答 ;用流式细胞仪及自动图象分析来检测细胞凋亡。结果　老年人淋巴细胞比青年人淋巴细胞的增殖应答能力低 ,CD4 5RA+细胞亚群减少 ,而CD4 5RO+细胞亚群增多 ,后者易发生凋亡。而中药成分PE对老年人淋巴细胞凋亡有抑制作用。结论　老年人淋巴细胞易于细胞凋亡 ,取决于活化 ,称为活化诱导性细胞死亡 ,此在免疫衰老中起着重要作用。

关 键 词：pollen extract LYMPHOCYTES APOPTOSIS CD_(45)RO^+ cells automatic imageanalysis 

分 类 号：R285[医药卫生—中药学]