原发性干燥综合征唇腺组织Fas、Fasl、Bcl-2表达的免疫组化研究  

The expression of Fas,Fasl and Bcl-2 in the minor labial salirary glandular destruction of patients with primary Siogren’s syndrome

作　　者：汤艳春[1] 王吉波[2] 

机构地区：[1]烟台毓璜顶医院,山东烟台264000 [2]青岛大学医学院附属医院

出　　处：《山东医药》2003年第28期13-15,共3页Shandong Medical Journal

摘　　要：目的　探讨 Fas、Fas配体 (Fasl)及 Bcl- 2在原发性干燥综合征 (PSS)患者唇腺组织中的表达、定位及其与 PSS组织损伤的关系。方法　采用免疫组织化学方法对 30例 PSS患者及 10例正常人组织唇腺中 Fas、Fasl及 Bcl- 2的表达进行检测和组织细胞定位。结果　 PSS患者唇腺组织腺泡上皮细胞 (AEC)和导管上皮细胞(DEC)中 ,Fas/ Fasl表达阳性细胞百分比明显增加 ,与正常对照组比较有高度显著差异 (P <0 .0 1) ;Bcl- 2表达阳性细胞百分比明显减少 ,与正常对照组比较有显著差异 (P <0 .0 5 )。 PSS组 AEC、DEC及浸润单核细胞(IMC)中 ,Fas表达阳性细胞百分比三者间比较均无显著差异 (P >0 .0 5 ) ;Fasl表达阳性细胞百分比 DEC与IMC均显著高于 AEC(P<0 .0 5 ) ;Bcl- 2阳性细胞表达百分比 :IMC显著高于 AEC和 DEC(P<0 .0 5 )。结论　PSS患者唇腺上皮细胞 Fas、Fasl表达增加及 Bcl- 2表达减少可能是 PSS患者唾液腺破坏的原因之一 ;Fas、Fasl和 Bcl- 2在 IMC中异常表达也为Objectire To investigate the roles of expression and location of Fas,Fasl and Bcl-2 in the minor labial salivary glandular destruction of patients with primary Sjogren’s syndrome(PSS).Minor submucosal labial salivary gland biopsies from 30 patient with PSS and 10 normal controls were detected the expression of Fas,Fas ligand(Fasl) and Bcl-2 in situ by immunohistochemical technique.Results PSS acinar epithelial cells(AEC) and ductal epithelial cells(DEC)showed increased expression of Fas/Fasl and decreased expression of Bcl-2 compared with that in normal glands.Among PSS AEC,DEC and infiltrating mononuclear cells (IMC),the percentage of cells expressing Fasl and Bcl-2 was observed significant difference,whereas the percentage of cells expressing Fas wasn’t observed significant difference.Conclusion The expression of Fas/Fasl increasing and that of Bcl-2 decreasing in epithielial cells of the labial salivary glands may be one of the mechanisms leading to glandular destruction found in PSS;the expressions of Fas,Fasl and Bcl-2 in IMC may be also an important mechanism leading to glandular destruction found in PSS.

关 键 词：原发性干燥综合征 唇腺 Fas FASL Bcl-2 癌基因 免疫组织化学 FAS配体 

分 类 号：R442.8[医药卫生—诊断学]