益妇宁软胶囊对去势大鼠血清性激素、自由基及NO水平的影响  

Effects of Yi-Fu-Ning Soft Capsule on Sex Hormone,NO,NOS,SOD and MDA Levels in Ovariectomized Rat Serum

作　　者：肖炜[1] 邓虹珠[1] 马云[2] 陈育尧[1] 杨俊[1] 

机构地区：[1]第一军医大学中医系,广州510515 [2]第一军医大学南方医院,广州510515

出　　处：《中药材》2003年第10期740-744,共5页Journal of Chinese Medicinal Materials

基　　金：军队医药卫生科研基金课题(2 0 0 0B0 1);广东省中医药局项目(4 0 0 0 0 1)

摘　　要：目的 :通过观察去势大鼠血清性激素、NO、NOS、SOD、MDA水平变化 ,探讨绝经后心血管疾病发生机制及中药复方益妇宁软胶囊对上述指标的影响。方法 :5 0只SD雌性大鼠随机分为 5组 :假手术组、去卵巢对照组、己烯雌酚片组、益妇宁大、小剂量组。给药 4周后测定血清E2 、P、NO、NOS、SOD、MDA水平。结果 :大鼠去势后随着血清E2 浓度的下降 ,NO、NOS、SOD水平也下降 ,MDA水平升高 ,E2 与其它指标有相关性 ;益妇宁软胶囊能调节血清性激素 ,明显改善上述指标。结论 :绝经后心血管疾病发生可能由于血清雌激素的减少 ,NO减少和自由基损伤途径发病 ;益妇宁软胶囊可以通过改善上述指标 ,防治绝经后心血管疾病的发生。Objective:Through investigating the changs of sex hormone,nitric oxide (NO),nitric oxide synthase (NOS),superoxide dismutase (SOD) and malonaldehyde (MDA) in the serum of ovariectomized rats,the mechanism of cardiovascular disease occurring in post menopause rats and the effect of Yi Fu Ning soft capsule (YFN) on above menticned indexes were discussed, Method:Fifty female mature Sprague Dawley rats were randomly divided into 5 groups:sham operation, ovariectomy (OVX), OVX with diethylstilbestrol tablets (DT), OVX with YFN (high dose and low dose) After administrating drugs of 4 weeks, sex hormone,NO,NOS,SOD and MDA levels in rat serum were detected Result: In ovariectomized rats serum, with the drop of E 2 concentration, NO,NOS,SOD levels decreased,while MDA content increased And the E 2 concentration was correlated with other index levels YFN can obviously regulate above mentioned indexes Conclusion:The cardiovascular disease occurring in post menopause rats is possibly due to the decrease of serum sex hormone,which cause the disease through the loss of the NO and damage of the free radical YFN can prevent and cure the cardiovascular disease by regulating above mentioned indexes in post menopause rats

关 键 词：益妇宁软胶囊 去势大鼠 血清 性激素 自由基 NO 一氧化氮 一氧化氮合成酶 更年期综合征 

分 类 号：R285.5[医药卫生—中药学] R271.917.5[医药卫生—中医学]