葡萄糖负荷对细菌性兔骨关节炎动物模型摄取^(99)Tc^m(Ⅴ)-DMSA及其生物分布的影响  

Study of biologic distribution and uptake of ^(99)Tc^m(Ⅴ)-DMSA in bacterial osteoarthritis rabbit model under glucose loading

作　　者：武兆忠[1] 林伟[1] 魏学立[1] 邬恒夫[1] 吴长伟[1] 袁戈文[1] 

机构地区：[1]广州医学院附属第二医院核医学科,510260

出　　处：《中华核医学杂志》2003年第5期305-307,T001,共4页Chinese Journal of Nuclear Medicine

基　　金：广东省医学科研基金资助项目 (B1 9980 6 3)

摘　　要：目的　探讨葡萄糖负荷影响99Tcm(Ⅴ ) 二巯基丁二酸 (DMSA)在细菌性骨关节炎症处浓集的可能机制。方法　自制细菌性兔骨关节炎动物模型 ,葡萄糖负荷下分别用感兴趣区技术和百分注射量分析法 ,分析葡萄糖负荷对99Tcm(Ⅴ ) DMSA生物分布及骨关节炎症处摄取和滞留99Tcm(Ⅴ ) DMSA的影响。结果　葡萄糖负荷对99Tcm(Ⅴ) DMSA全身生物分布没有明显影响 ,明显提高细菌性骨关节炎症区99Tcm(Ⅴ ) DMSA摄取水平 ,其 6h患侧与健侧比值 :显像分析法为对照组 2 882± 0 52 5,实验组 3 487± 0 53 5(P <0 0 5) ;百分注射量分析法为对照组 3 153± 0 3 3 4 ,实验组 5 0 71± 1 780 (P <0 0 5)。结论　葡萄糖负荷可提高骨组织炎症区99Tcm(Ⅴ )Objective To study the possible mechanism of 99 Tc m(Ⅴ)-dimercaptosuccinic acid (DMSA) localization in the bacterial osteoarthritis under glucose loading. Methods In bacterial osteoarthritis rabbit model loaded by glucoce, the methods both the region of interest technique and the percentage of injected dose were used to analyze the influence of biologic distribution of 99 Tc m (Ⅴ)-DMSA and uptake of 99 Tc m(Ⅴ)-DMSA in osteoarthritic bone tissues. Results There was no influence of whole body biologi c distribution of 99 Tc m(Ⅴ)-DMSA under glucose loading, but the uptake of 99 Tc m(Ⅴ)-DMSA in the osteoarthritic bone tis sues was remarkably increased, the lesion to no-lesion ratio of 99 Tc m(Ⅴ)-DMSA 6 h post injection: ①SPECT scan method were 2.882±0.5 25 in control group and 3.487±0.535 in glucose loading group( P <0.05); ② percentage injection dose method were 3.153±0.334 in control group and 5.071±1 .780 in glucose loading group( P <0.05). Conclusion Glucose can increase the uptake of 99 Tc m(Ⅴ)-DMSA in the osteoarthriti c bone tissues.

关 键 词：葡萄糖负荷 细菌性骨关节炎 动物模型 ^99Tc^m(Ⅴ)-DMSA 生物分布 兔 ^99Tc^m(Ⅴ)-二巯基丁二酸 放射性核素显像 

分 类 号：R817[医药卫生—影像医学与核医学]