Vascular endothelial growth factor gene transfection to enhance the repair of avascular necrosis of the femoral head of rabbit  

作　　者：杨操 杨述华 杜靖远 李进 许伟华 熊宇芳 

机构地区：[1]Department of Orthopedics,Union Hospital,Tongji Medical College,Huazhong University of Science & Technology [2]Department of Biochemistry,Tongji Medical College,Huazhong University of Science & Technology

出　　处：《Chinese Medical Journal》2003年第10期1544-1548,共5页中华医学杂志（英文版）

基　　金：ThisstudywassupportedbyagrantfromtheNationalNaturalScienceFoundationofChina (No 3 0 170 945 )

摘　　要：Objective To explore a new method for the therapy of avascular necrosis of the femoral head. Methods The recombinant plasmid pCD-hVEGF 165 was mixed with collagen and was implanted in the necrotic femoral head. The expression of vascular endothelial growth factor (VEGF) was examined by RNA dot hybridization and immunohistochemical techniques. Repair of the femoral head was observed by histological and histomorphometric analysis.Results The expression of VEGF was detected in the femoral head transfected with the VEGF gene. The femoral head transfected with the VEGF gene showed a significant increase in angiogenesis 2 and 4 weeks after gene transfection and a significant increase in bone formation 6 and 8 weeks after gene transfection on histomorphometric analysis ( P <0.01).Conclusions Transfection of the VEGF gene enhances bone tissue angiogenesis. Repair of osteonecrosis could be accelerated accordingly,thus providing a potential method for therapy of osteonecrosis.Objective To explore a new method for the therapy of avascular necrosis of the femoral head. Methods The recombinant plasmid pCD-hVEGF 165 was mixed with collagen and was implanted in the necrotic femoral head. The expression of vascular endothelial growth factor (VEGF) was examined by RNA dot hybridization and immunohistochemical techniques. Repair of the femoral head was observed by histological and histomorphometric analysis.Results The expression of VEGF was detected in the femoral head transfected with the VEGF gene. The femoral head transfected with the VEGF gene showed a significant increase in angiogenesis 2 and 4 weeks after gene transfection and a significant increase in bone formation 6 and 8 weeks after gene transfection on histomorphometric analysis ( P <0.01).Conclusions Transfection of the VEGF gene enhances bone tissue angiogenesis. Repair of osteonecrosis could be accelerated accordingly,thus providing a potential method for therapy of osteonecrosis.

关 键 词：vascular endothelial growth factor gene transfection avascular necrosis femoral head 

分 类 号：R681.8[医药卫生—骨科学] R394[医药卫生—外科学]