胎儿和少儿皮肤内转化生长因子-β1和β3基因表达的变化  

Gene expression of transforming growth factor-β1,β3 in fetal and children skin

作　　者：陈伟[1] 付小兵[1] 葛世丽[2] 周岗[1] 盛志勇[1] 

机构地区：[1]解放军304医院全军创伤修复重点实验室,北京100037 [2]军事医学科学院放射医学研究所

出　　处：《中华实验外科杂志》2003年第11期970-972,共3页Chinese Journal of Experimental Surgery

基　　金：国家重大基础研究规划资助项目 (G1 9990 542 0 4 );国家自然科学基金重点项目 (30 2 30 370 )

摘　　要：目的　探讨转化生长因子 β1(TGF β1) ,TGF β3及其Ⅰ 型和Ⅱ 型受体 (TBRⅠ ,TBRⅡ )在胎儿和少儿皮肤中表达的特征。方法　提取 18例不同胎龄的胎儿皮肤和 6例少儿皮肤的总RNA后 ,分离mRNA ,用逆转录 多聚酶链反应 (RT PCR)方法检测这 4种基因的表达。结果　在早期妊娠胎儿的皮肤中 ,TGF β1,TBRⅠ和TBRⅡ基因表达较弱 ,随着胎龄的增加 ,这 3种基因表达逐渐增强。在少儿皮肤内 ,这 3种基因的表达量分别为妊娠早期皮肤的 1.3 ,1.3和 1.2倍 ,基因表达显著升高 (P <0 .0 5 )。在早期胚胎的皮肤中 ,TGF β3基因表达较强 ,而在少儿皮肤内 ,该基因表达低下。结论　在早期胚胎皮肤中 ,TGF β1,TBRⅠ和TBRⅡ基因低表达 ,TGF β3基因的高表达可能与胎儿皮肤创面无瘢痕修复密切相关。Objective To investigate the gene expression of transforming growth factor-β1 (TGF-β1),TGF-β3,and their two receptors (TBRⅠand TBRⅡ) in fetal and children skin.Methods The gene expression of TGF-β1,TGF-β3,TBRⅠand TBRⅡ in skin at different developmental stages was detected by using reverse transcription-polymerase chain reaction (RT-PCR) assay.Results In early gestational fetal skin,the gene expression of TGF-β1,TBRⅠand TBRⅡ was weaker.Along with the increment in gestational age,the gene expression of these 3 genes was elevated.In children skin,the expression levels of these 3 genes were 1.3,1.3,1.2 folds of early gestational skin,respectively.In marked contrast,the content of TGF-β3 gene transcript was higher in early gestational skin than in late gestational and children skin (P<0.05).Conclusion The relative lack of gene expression of TGF-β1,TBRⅠand TBRⅡ and increment of TGF-β3 gene expression in skin of younger fetuses might be involved in fetal scarless healing.

关 键 词：胎儿 少儿 皮肤 转化生长因子-Β1 转化生长因子-Β3 基因表达 转化生长因子受体 

分 类 号：R64[医药卫生—外科学]