11,12-环氧二十碳三烯酸预处理对未成熟兔离体心脏的影响  

Effect of 11,12-epoxyeicosatrienoic acid preconditioning on the isolated immature rabbit heart

作　　者：周明阳[1] 吴清玉[1] 龙村[2] 温复兴[2] 史世勇[2] 覃筱燕[3] 

机构地区：[1]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院心外科,北京100037 [2]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院麻醉与体外循环研究室,北京100037 [3]中央民族大学生物化学系

出　　处：《中华实验外科杂志》2003年第11期1009-1010,共2页Chinese Journal of Experimental Surgery

基　　金：中国医学科学院重点基金资助项目 (0 2 1 98)

摘　　要：目的　观察 11,12 环氧二十碳三烯酸 ( 11,12 EET)预处理对离体未成熟兔心肌缺血再灌注的保护作用 ,并探讨其可能机制及意义。方法　利用Langendorff灌注装置 ,建立心肌缺血再灌注模型。 2 4只未成熟兔随机分成对照组、缺血预处理组 (IPC组 )、11,12 EET预处理组 (EET组 ) ,观察常温缺血 ( 3 7℃ ) 2h再灌注 1h前后心功能变化 (左室变化压、左室舒张末期压、心室最大压力变化速率、冠状动脉流量等指标 )、心肌含水量、心律失常评分、心肌酶学 [肌酸激酶 (CK )、乳酸脱氢酶 (LDH ) ]指标。结果　IPC组及EET组心功能恢复、心律失常发生及心肌酶学指标均优于对照组 ;与IPC组相比 ,EET组的心肌含水量 ( 75 .3± 3 .5 ) %、CK( 117.4± 8.3 )IU/L、LDH ( 5 2 .7±13 .4)IU/L均较低 (P <0 .0 5 ) ,冠状动脉流量 ( 12 .7± 1.5 )ml则较大 ,这两组的其余观察指标差异无显著性 (P >0 .0 5 )。结论　 11,12 EET预处理未成熟兔离体心脏能产生优于缺血预处理的心肌保护作用。Objective To study the effectiveness of 11,12-epoxyeicosatrienoic acid (11,12-EET) preconditioning in protecting myocardium of immature hearts from ischemic reperfusion injury.Methods On the Langendorff perfusion apparatus,isolated perfused immature rabbit hearts underwent 120 min of normothermic ischemia,followed by 60 min of reperfusion.Twenty-four isolated immature rabbit hearts were divided into three groups randomly: control group,ischemic preconditioning (IPC) group and 11,12-EET preconditioning (EET) group.The preischemia and postischemia myocardial function was assessed by the percentage recovery of left ventricle developed pressure (LVDP),left ventricle end-diastolic pressure (LVEDP),±dp/ptmax,myocardial water content (MWC),and coronary blood effluent (CBE).The index of arrhythmia scores (AS),CK and LDH were also observed.Results The indexes of myocardial function,AS,CK and LDH in IPC and EET groups were significantly better than control group.The values of CK (117.4±8.3) IU/L,LDH (52.7±13.4) IU/L and MWC (75.3±3.5)% in EET group were lower than in IPC group.At the same time,CBE (12.7±1.5) ml was increased significantly.Conclusion 11,12-EET is a superior preconditioning agent to IPC.

关 键 词：11 12-环氧二十碳三烯酸 预处理 未成熟离体心脏 兔 心肌保护 心肌缺血 再灌注损伤 

分 类 号：R965[医药卫生—药理学]