联合应用抗菌药物治疗创伤弧菌感染的实验研究  

The combination experimental therapy with antimicrobial agents in Vibrio vulnif icus infection in mouse

作　　者：卢中秋[1] 张秀华[2] 周铁丽[3] 胡国新[4] 程俊彦[1] 李景荣[1] 

机构地区：[1]温州医学院附属第一医院急诊科,325000 [2]温州医学院附属第一医院药剂科,325000 [3]温州医学院附属第一医院细菌室,325000 [4]温州医学院附属第一医院药理教研室,325000

出　　处：《中华传染病杂志》2003年第5期312-315,共4页Chinese Journal of Infectious Diseases

基　　金：浙江省教育厅基金资助项目(2 0 0 2 0 4 38)

摘　　要：目的　探讨联合应用抗菌药物对创伤弧菌感染小鼠的治疗效果。方法　创伤弧菌 ( 6.0× 10 8cfu/ml,0 .6ml/10g小鼠 )经腹腔注射感染小鼠后 1h、2h、3h ,分别以人体常用治疗量的抗菌药物亚胺培南、氯霉素、多西环素 (灌胃 )、奈替米星、头孢哌酮、哌拉西林、左旋氧氟沙星等单独或联合用药 ,观察存活小鼠数及小鼠脏器的超微结构变化。结果　创伤弧菌感染早期上述 7种抗菌药物均有显著治疗作用 ,氯霉素、奈替米星、头孢哌酮、左旋氧氟沙星疗效最好 ,亚胺培南治疗效果较差。头孢哌酮 +左旋氧氟沙星、头孢哌酮 +奈替米星、奈替米星 +多西环素联用能显著增加治疗效果 ,小鼠主要脏器超微结构损伤恢复正常。结论　及早选用氯霉素、奈替米星、头孢哌酮、左旋氧氟沙星对创伤弧菌感染小鼠有较好的治疗效果 ,头孢哌酮与左旋氧氟沙星、头孢哌酮与奈替米星。Objective We conducted an in vivo study with the mouse model of Vib rio vulnificus infection to evaluate the efficacies of combination therapy with antimicrobia l agents. Methods Vibrio vulnificus (6.0×10 8 cfu/ml)was injected intraperitoneally into the right abdominal cavity. One hour, 2 hour and 3 hour after inoculation, 7 antimicrobial agents were given alone or in combination intraperitoneally at human therapeutic dose level. The numbers of survial mouse and the supermicrostr ucture change of organs w ere observed. 7 antimicrobial agents were Imipenem, Chloramphenicol, Doxycycline Hydrochloride, Netilmicin, Cefoperazone, Piperacillin, Levofloxacin. Res ults Two hours after infection, the mouse survival rates of groups trea ted with Chloramphenicol, Levofloxacin, Netilmicin, Cefoperazone was 100%. Howev er, the survival rates in the mouse treated by Piperacillin was 60% and the surv ival rate of in the mouse treated with Imipenem or Doxycycline Hydrochloride was 20%. The survival rate in the mouse treated with Cefoperazone combined with Lev ofloxacin, Cefoperazone combined with Netilmicin, or Netilmicin combined with Do xycycline Hydrochloride, was 100%. The supermicrostructure injure of the organs in the mouse recovered. Conclusions These results indicate that Chloramphenicol, Netilmicin, Cefoperazone and Levofl oxacin alone had satisfactory efficacy in the treatment of experimental Vibrio v ulnificus infection in mouse. The combination therapies of Cefoperazone with Lev ofloxacin, Cefoperazone with Netilmicin, and Netilmicin with Doxycycline Hydroch loride are more advantageous than using antimicrobial agent alone.

关 键 词：联合用药 抗菌药物 药物治疗 创伤 弧菌感染 实验 

分 类 号：R516[医药卫生—内科学]