复圣散对高脂大鼠脑缺血再灌后脑内NPY含量及其mRNA表达的影响  

Effect of Fu-Sheng powder on NPY and its mRNA in brain of rats with hyperlipidemia after cerebral ischemia and reperfusion

作　　者：雷燕[1] 黄启福[2] 王永炎[3] 

机构地区：[1]中国中医研究院西苑医院,北京100091 [2]北京中医药大学脑病研究室,北京100029 [3]中国中医研究院,北京100700

出　　处：《中国病理生理杂志》2001年第7期652-654,共3页Chinese Journal of Pathophysiology

基　　金：中国博士后科学基金资助项目 (No .0 5 432 )

摘　　要：目的 :探讨脑缺血后内源性缩血管活性肽神经肽Y(NPY)的变化规律以及中药复圣散对其影响。方法 :通过高脂饮食喂养复制食饵性高脂血症大鼠 ,在此基础上采用反复脑缺血再灌流手术造成大鼠脑损伤模型 ,以放免法测定高脂大鼠脑缺血再灌注后脑内NPY含量的动态变化 ,并运用原位杂交技术观察模型组大鼠脑组织NPYmRNA表达模式。结果 :脑缺血再灌后 1d模型组大鼠脑内NPY含量比正常对照组高 5 1 86 % (P <0 0 5 ) ,并持续至术后 7d ,且NPYmRNA表达上调 ,而复圣散预防和治疗性给药组大鼠脑内NPY含量明显少于同期模型组 (P <0 .0 5 ) ;同时 ,复圣散预防和治疗组大鼠脑内NPYmRNA表达下调。结论 :提示脑缺血性损伤与脑内NPY释放失衡密切相关 ,而中药复圣散对脑内神经肽Y含量有一定的调节作用 ,推测这可能是该方防治脑血管病的主要作用机制之一。AIM: To observe the changes in neuropeptide Y(NPY) and the effect of Fu-Sheng powder(FSP) on NPY in the rat brain in a steady cerebral ischemia and reperfusion(I/R) model. METHODS: The models of rat brain injury were established by repeated cerebral I/R in rats with hyperlipidemia. Radioimmunoassay was performed to determine the level of NPY, while NPY mRNA expression was observed by in situ hybridization. RESULTS: After 1 day of I/R, compared with control group, the content of NPY in the model animals were significantly increased by 51.86% ( P< 0 01) and lasting 7 days after I/R, and the expression of NPY mRNA was greatly increased. FSP treatment decreased the contents of NPY ( P< 0 05, P< 0 01) and its mRNA expression. CONCLUTION: There were obvious imbalances of NPY in the rat brain after cerebral I/R and the FSP might antagonize ischemia injury of brain through modulating NPY, which may be one of the mechanisms underlying FSP treatment for cerebral vascular diseases. [

关 键 词：复圣散 高脂 大鼠 脑缺血 再灌注损伤 MPY MRNA表达 中药 内源性缩血管活性肽神经肽Y 

分 类 号：R277.733.1[医药卫生—中医学] R285.5