复方大黄制剂预防大鼠肥胖的实验研究  

Experimental study on preventing obesity by compound rhubarb preparation in rats

作　　者：曲晓义[1] 陈愉[1] 金惠铭[1] 焦东海 宋海林 

机构地区：[1]复旦大学医学院病理生理学教研室,上海200032 [2]上海市香山中医院及上海市中西医结合肥胖病医疗协作中心,上海200020

出　　处：《中国病理生理杂志》2001年第7期673-675,共3页Chinese Journal of Pathophysiology

基　　金：上海市促进高新科技成果转化项目 (No 99-0 2 5 5 )

摘　　要：目的 :研究复方大黄制剂预防大鼠肥胖的效果及其可能机制。方法 :新生鼠 2 6只 ,随机分成大黄制剂 +高能饲料组 (n =8)、高能饲料对照组 (n =8)和普通饲料对照组 (n =10 )。大黄制剂 +高能饲料组和高能饲料对照组喂高能量饲料 ,普通饲料对照组喂普通饲料。第 9周后大黄制剂 +高能饲料组给予复方大黄制剂灌服 ,剂量 40mg·10 0g-1bodyweight·d-1,给药 8周 ,观察大鼠体重动态变化 ,腹腔脂肪重量 ,脂肪细胞大小 (显微电脑测量 ) ,脂肪细胞中瘦素 (leptin)表达 (ABC法 ) ,血清瘦素水平 (放免法 )变化。结果 :大黄制剂 +高能饲料组大鼠体重明显低于高能饲料对照组 ,脂肪重量明显轻于高能饲料对照组 ,脂肪细胞小于高能饲料对照组 ,脂肪细胞周围瘦素表达明显弱于高能饲料对照组 (灰度值 ) ,且与腹腔脂肪组织重量间呈显著正相关 (r=0 86 6 3,P <0 0 5 ) ,而血清瘦素水平变化不大。结论 :复方大黄制剂 (40mg·10 0g-1bodyweight·d-1)对大鼠肥胖有预防作用 ,其机制可能与脂肪细胞瘦素表达减弱有关。AIM: To investigate the effect of compound rhubarb preparation (Kintop) in preventing obesity in rats and its probable mechanism involved. METHODS: Twenty-six newborn SD rats were randomly grouped as rhubarb preparation plus high-energy forage group( n= 8), high-energy forage control group( n= 8) and ordinary forage control group( n= 10). The rats in rhubarb preparation plus high-energy forage group and high-energy forage control group were fed with high-energy forage and those in ordinary forage control group were fed with ordinary forage. The rats in rhubarb preparation plus high-energy forage group were administered by compound rhubarb preparation (40 mg·100 g -1 body weight·d -1 ) from 9th to 17th week. The dynamic changes in body weight, celiac fat weight and adipocytes size were measured. Immunohistochemical analysis of leptin in celiac adipocytes (ABC method) and measurement of serum leptin level (RID method) were performed. RESULTS: The body weight and the wet weights of celiac fat were lower, their adipocytes were smaller and immunohistochemical stainings of leptin were weaker in rhubarb preparation plus high-energy forage group than those in high-energy forage control group. There was an obvious positive correlation between the expression of leptin and celiac fat tissue weight( r= 0.8663, P< 0.05). But the changes in serum leptin were not significant between groups. CONCLUSION: Compound rhubarb preparation (40 mg·100 g -1 body weight·d -1 ) can prevent the obesity in rats fed with high-energy forage. The mechanism might involve the decrease in adipocyte leptin expression. [

关 键 词：复方大黄制剂 预防 大鼠 肥胖 实验 中药 

分 类 号：R259.892[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]