成人骨髓间质干细胞定向诱导为脂肪细胞的研究  

Adult human mesenchymal stem cell differentiates into adipocytes

作　　者：项鹏[1] 张丽蓉[2] 陈振光[3] 夏文杰[1] 张秀明[1] 李艳[1] 李树浓[1] 

机构地区：[1]中山医科大学病理生理教研室,广东广州510080 [2]广东药学院病理生理教研室,广东广州510224 [3]中山医科大学附属第一医院心胸外科,广东广州510080

出　　处：《中国病理生理杂志》2001年第7期598-601,T001,共5页Chinese Journal of Pathophysiology

基　　金：国家重点基础研究发展规划项目 (973)资助(No .G19990 5 430 0 )

摘　　要：目的 :体外定向诱导成人骨髓间质干细胞 (MSC)分化为脂肪细胞。方法 :采用Ficoll-Paque淋巴细胞分离液 (1 0 77× 10 3 g/L)离心分离成人MSC ,体外扩增 ,流式细胞仪检测MSC表面抗原表达 ,地塞米松、IBMX、胰岛素、indomethacin定向诱导MSC分化为脂肪细胞。油红O检测中性脂肪 ,光镜下细胞计数。结果 :成人骨髓间质干细胞在体外扩增 5代可获得 1× 10 8个细胞。流式细胞仪检测结果显示CD2 9、CD44、CD90、CD10 5、CD16 6表达阳性 ,CD14、CD34、CD45、CD11a为阴性。诱导 48h后 ,细胞内有脂滴出现 ,随着时间延长 ,脂滴逐渐增加并融合为脂泡。细胞由梭形转变为圆形或多角性。细胞计数结果显示 85 %以上细胞转变为脂肪细胞。结论 :成人骨髓间质干细胞在体外可以分化为脂肪细胞。AIM: To investigate the differentiation from human mesenchymal stem cells (hMSC) to adipocytes.METHODS: hMSC were separated from rib marrow and expanded in culture medium. To detect the surface antigens, the labeled cells were analysed on a FACScan flow cytometer. hMSC were induced with dexamethasone, insulin, 1-methy1-3-isobutylxanthine and indomethacin which acted as adipocyte differentiation inducer. The cells were stained with Oil Red O. The number of adipocytes were counted on a phase-contrast microscope.RESULTS: hMSC were expanded as undifferentiated cells in culture for more than 5 passages. The isolated cultured MSC comprised a single phenotypic population and displayed a fibroblast-like morphology. These expanded, attached MSC were uniformly positive for CD29, CD44, CD90, CD105, CD166 and didn't express CD14, CD34, CD45, CD11a. After induced with induction medium, lipid vacuoles were first detectable within the cells at 48 hours. Two weeks later, more than 85% MSC differentiated into adipocytes which displayed a perinuclear accumlation of lipid vacuoles, as detected by Oil Red O. CONCLUSION: hMSC can be induced to differentiate into adipocytes. [

关 键 词：成人骨髓间质干细胞 脂肪细胞 肥胖症 造血干细胞 

分 类 号：R589.2[医药卫生—内分泌] R329.2[医药卫生—内科学]