组织原位杂交法研究输血传播病毒的致病性  

Pathogenecity and replication of transfusion transmitted virus in liver tissue by in situ hybridization

作　　者：阎惠平[1] 郎振为[1] 黄德庄[1] 周育森[2] 金荣华[1] 罗朝霞[1] 贺立香[1] 王海涛[2] 

机构地区：[1]北京佑安医院北京市肝炎研究所分子生物学室,100054 [2]军事医学科学院五所

出　　处：《中华预防医学杂志》2001年第1期10-12,共3页Chinese Journal of Preventive Medicine

基　　金：北京市自然科学基金资助!(7992 0 2 3)

摘　　要：目的　根据肝组织中TT病毒 (TTV)感染和复制状况探讨病毒的致病性。方法　以重组质粒为模板、地高辛素为标记物、对称和不对称PCR法分别标记TTV双链探针和 2条单链探针。原位杂交检测 5 6例肝组织 ,其中 6例TTV阳性者分别做单链探针杂交。结果　 5 1例非甲～非庚型肝炎肝组织中TTV阳性率 2 7 5 % (14/5 1)。对照组 5例杂交阴性。TTVDNA主要见于肝细胞核内 ,受染细胞的病变和坏死不十分明显 ;在急性、慢性和重症肝炎及肝硬化组织中均有检出。用单链探针检测病毒基因链的结果与双链探针杂交结果一致 ,杂交信号强度相仿 ;2例肝组织TTV基因链与互补链同时存在 ,信号强度较弱。结论　TTV可能导致肝损伤 ,并在肝脏内有复制 ;Objective To study its pathogenecity based on infection status and replication of transfusion transmitted virus(TTV)in the liver tissues Methods Using recombinant plasmid as template and symmetrical and asymmetrical polymerase chain reaction (PCR),double strand and single strand TTV probes were labeled with digoxigenin Fifty six cases of liver biopsy specimens were detected by in situ hybridization,and six positive cases of them were tested further with single strand probes Results TTV DNA was found in 14 of 51 patients with non A and non G hepatitis (27 5%),and five cases in the control group were negative TTV DNA was mainly observed in the nuclei of hepatocytes and pathologic changes and necrosis in the infected liver cells were not so apparent,but they could be seen in the liver tissues of acute,chronic and severe hepatitis,as well as of liver cirrhosis TTV genome strand hybridization detected by single strand probe was consistent with that by double strand probe Both TTV genome strand and its complementary strand were present in the two cases of liver specimens,but hybridizational signals were weaker in the latter than those in the former Conclusion TTV could cause injury and replicate itself in the liver,but no obvious and direct injury to hepatocytes

关 键 词：组织原位杂交法 输血 病毒传播 致病性 TT病毒感染 聚合酶链法 

分 类 号：R457[医药卫生—治疗学]