纳米羟晶/胶原仿生骨修复家兔颅颌骨缺损的实验研究  

The repair of bony defects in rabbits by implanting nacre-minicking structural hydroxyapatite/collagen composite

作　　者：姚晖[1] 杜昶[2] 杨韶华[1] 崔福斋[2] 雷涛[1] 

机构地区：[1]天津市口腔医院生物医学工程研究室,天津300041 [2]北京清华大学材料科学与工程系,北京100084

出　　处：《透析与人工器官》2000年第2期5-8,共4页Chinese Journal of Dialysis and Artificial Organs

摘　　要：目的 :采用先进的仿生骨材料制备工艺 ,摹仿天然骨骼的形成过程 ,制备具有天然骨中纳米尺寸的羟基磷灰石 /胶原复合人工骨材料 ,并用其修复家兔颅颌骨实验性穿通型骨缺损。方法 :用四环素荧光示踪 ,放射性同位素 45 Ca Cl2 标记和放射自显影等手段观察和评价骨创的愈合情况 ,结果 :该新型仿生骨材料具有良好的生物相容性 ,促进和加快骨创愈合的作用明显 ,为一种颇有前途的颅颌面骨缺损修复材料。Objective:[WT5”BZ]It is well known that HAP is nanometre sized and deposits in an orderly way on the collagen matrix in natural bones of the body.However,with the synthetic methods available,it is not easy to obtain either a uniformaly mixed composite,or a fine crystal sized material,while these two factors are important to the bioactivities of the composite.The present study used a new preparation method of HAP/Collagen Composite,in which nanometre sized HAP was homogeneously dispersed in collagen matrix,and then implanted the composite into the experimental bone defects in 4 rabbits,With same amount of control defects site in which no material was implanted.The healing effects of bone defects were assessed by methods of Tetracycline fluorescent label,radioisotope tracer and autoradiography.The morphologies of the HAP/Collagen Composite showed that HAP precipitates were uniformly distributed on the collagen matrix in the HAP/Collagen Composite and the particle dimension is only 2～10 nm.Therefore,the HAP in the HAP/Collagen Composite is nanometre sized.The histological observation showed that this new bionic bony material has good biocompatible and better bioactivities.It could stimulate the osteoblastic cells and accelerate the healing of the bony defect.It is concluded that the newly developed composite is a hopeful boneimitating material (bionic bony material)for oral maxillofacial implantation. [WT5”HZ]

关 键 词：纳米羟晶 胶原 仿生骨 家兔 颅颌骨缺损 荧光示踪 放射性同位素标记 控制析出法 

分 类 号：R318.17[医药卫生—生物医学工程]