卡他莫拉菌的检测及药物敏感性  

Detection and Susceptibility of Moraxella catarrhalis

作　　者：胡付品[1] 朱德妹[1] 李胜利[1] 吴湜[1] 张婴元[1] 

机构地区：[1]复旦大学附属华山医院抗生素研究所,上海200040

出　　处：《中国抗感染化疗杂志》2001年第4期218-221,共4页Chinese Journal of Infection and Chemotherapy

摘　　要：目的:以DNA酶试验鉴定结果为标准,建立聚合酶链反应(PCR)鉴定卡他莫拉菌的方法,并测定抗菌药物对卡他莫拉菌的最低抑菌浓度(MIC).方法:PCR扩增卡他莫拉菌特异性片段,DNA酶试验检测卡他莫拉菌所产生的DNA酶,琼脂稀释法测定抗菌药物对卡他莫拉菌的MIC及头孢硝噻吩法检测β内酰胺酶.结果:在68株疑为卡他莫拉菌的临床分离菌株中,有48株细菌DNA酶试验阳性, 此48株细菌PCR结果亦为阳性;20株DNA酶试验阴性菌株PCR结果亦阴性,PCR检测和DNA酶检测符合率达100 %(68/68).β内酰胺酶产生率为95.8 %(46/48).2株不产酶卡他莫拉菌对受试的7种抗菌药物均显示敏感,药物的MIC均≤0.06 mg/L;氨苄西林等7种抗菌药物对46株产酶卡他莫拉菌的MIC50和MIC90范围分别为≤0.06～2 mg/L和0.25～4 mg/L.结论:PCR检测方法具有方便、快速及高特异性的特点,可作为检测卡他莫拉菌的辅助方法之一;卡他莫拉菌产酶株对酶抑制剂复方制剂、第三代头孢菌素、喹诺酮类及米诺环素均显示敏感.To establish the polymerase chain reaction(PCR) methed for detecting Moraxella catarrhalis with DNase testas reference and determine minimum inhibitory concentration(MIC) of antimicrobial agents against M. catarrhalis. Spe-cific fragment of M. catarrhalis DNA were amplified by PCR. DNase of M. catarrhalis was detected by DNase test. MICs of an-timicrobial agents against Moraxella catarrhalis were determined by agar dilution method and β-lactamases were detected by nitro-cefin test. The range of MIC50 and MIC90 of seven antibiotics on 46 β-1actamases-producing strains of M. catarrhalis were ≤0.06 -2mg/L, 0.25-4mg/L respectively. DNase test and PCR were both opsitive in 48 of 68 c1inica isolates and the other 20strains were all negative, the results of PCR were all the same as DNase test in detecting M.catarrhalis. The incidence ofβ-lacta-mases in M. cutarrhalis was 95.8 % (46/48). Two β-lactamases-negative M. catarrhalis strains were susceptible to seven antibi-otics tested and the MICs were both lower than 0.06mg/L.PCR method can be used to identify M. catarrhalis withadvantages of rapidity, specificity and easy to perform.β-1actamases-producing M. catarrhalis strains were susceptible toβ-lactam/β-lactamase inhibitor combination, the third-generation cephaloaporins, quinolones and minocycline.

关 键 词：卡他莫拉菌 检测 药物敏感性 聚合酶链反应 DNA酶试验 最低抑菌浓度 

分 类 号：R446.5[医药卫生—诊断学]