鲤和团头鲂幼鱼卵黄蛋白原的诱导、纯化及电泳比较  

INDUCTION,PURIFICATION AND ELECTROPHORETIC COMPARISON OF VITELLOGENIN FROM TWO CYPRINID FISH SPECIES:CARP (CYPRINUS CARPIO) AND WUCHANGFISH (MEGALOBRAMA AMBLYCEPHALA YIH)

作　　者：梁勇[1,2] 徐盈[1,2] 杨方星[1,2] 王剑伟[1,2] 

机构地区：[1]中国科学院水生生物研究所 [2]淡水生态与生物技术国家重点实验室,武汉430072

出　　处：《水生生物学报》2002年第4期317-321,共5页Acta Hydrobiologica Sinica

基　　金：中国科学院知识创新项目KZCX 4 1 0资助

摘　　要：采用腹腔注射人工雌激素 1 7α 乙炔基雌二醇 (1 7α ethinyloestradiol,EE2 )的方法 ,在鲤幼鱼和团头鲂幼鱼体内诱导生成卵黄蛋白原 ;并结合利用阴离子交换介质DEAE SephraroseCL 6B和液相层析技术分离纯化了诱导后的鲤幼鱼和团头鲂幼鱼血浆中的卵黄蛋白原 ;SDS PAGE电泳结果表明所分离的两种鲤科鱼类卵黄蛋白原其亚基的分子量分别为 1 70KD和1 5 0KD。Vitellogenin was induced in immature common carp (Cyprinus carpio L.) and immature Wuchangfish (Megalobrama amblycephala Yih) by intraperitoneal injection of xenobiotic estrogen 17α-ethinyloestradiol. By using the anion-exchange chromatography on a DEAE-Sephrarose CL-6B column, the vitellogenin in plasma from two cyprinid fish species was effectively purified. The results of SDS-PAGE show that the molecular weights of vitellogenin monomer in two cyprinid fish species are around 170KD and 150KD respectively.

关 键 词：卵黄蛋白原 鲤科 鱼类 阴离子交换 内分泌干扰物 团头鲂 

分 类 号：Q959.468[生物学—动物学]