食管鳞状细胞癌组织中细胞胀亡与凋亡检测  

Detection of oncosis and apoptosis in esophageal squamous cell carcinoma

作　　者：赵高峰[1] 僧靖静[1] 卫战文 田川[1] 高冬玲[3] 陈奎生[3] 马长路[3] 李全荣[4] 齐宇 马继红[1] 屈清荣[1] 张云汉[3] 

机构地区：[1]郑州大学第一附属医院外科,郑州450052 [2]孟州市中医院外科,孟州454750 [3]郑州大学第一附属医院病理科,郑州450052 [4]吴川市人民医院外科,吴川524500

出　　处：《郑州大学学报（医学版）》2003年第6期854-857,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南省杰出青年科学基金资助项目 0 3 12 0 0 2 3 0 0

摘　　要：目的 :探讨食管鳞状细胞癌组织中细胞胀亡与凋亡的存在及 2者间的关系。方法 :收集 30例食管鳞状细胞癌标本 ,利用透射电镜、TUNEL及免疫组织化学技术检测其中的胀亡、凋亡细胞及微血管密度 (MVD)。结果 :食管鳞状细胞癌组织中存在胀亡及凋亡细胞 ,凋亡指数 (AI)和胀亡指数 (OI)分别为 (1 .7± 1 .2 )和 (3.2± 1 .9)。与MVD低表达组比较 ,高表达组OI较低 ,而AI较高 ,差异有统计学意义 (P <0 .0 0 1或P <0 .0 0 5 )。结论 :食管鳞状细胞癌存在凋亡和胀亡两种细胞死亡方式 ,2者的存在及分布与MVD有关。Aim: To investigate the interrelationship of oncosis and apoptosis in esophageal carcinoma. Methods: Using transmission electron microscope, TUNEL, and immunohistochemical technique, the occurrence of oncosis, apoptosis, and microvessel density (MVD) in the esophageal carcinoma was determined. Results: Oncosis and apoptosis existed in esophageal squamous cell carcinoma. The apoptosis index(AI) and oncosis index(OI) were (1.7±1.2) and (3.2± 1.9) , respectively. Compared with MVD low-expressed group, OI was lower while AI was higher in the MVD high-expressed group( P <0.001 or 0.005). Conclusion:Esophageal squamous cell carcinoma presented oncosis and apoptosis. The occurrence and distribution of oncosis and apoptosis had close relationship with microvessel density.

关 键 词：食管肿瘤 鳞状细胞癌 细胞胀亡 凋亡 

分 类 号：R765.3[医药卫生—耳鼻咽喉科]