香蕉分生小球体途径胚性细胞悬浮系的建立  

Establishment of Embryogenic Cell Suspensions from Meristematic Globules of Musa spp

作　　者：徐春香[1] 李华平[1] 肖火根[1] 范怀忠[1] 

机构地区：[1]华南农业大学资源学院植物病毒研究室

出　　处：《园艺学报》2003年第5期580-582,共3页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(3 9870 43 4 );广东省自然科学基金项目(990 714 )

摘　　要：以 7个香蕉品种未成熟雄花为外植体均获得了分生小球体 (meristematicglobules)。分生小球体的诱导率因基因组型和品种而异 ,为 15%～ 81% ,分生小球体的特点及开始出现的时间也受基因组型和品种的影响。以分生小球体为起始材料 ,建立了‘广东 2号’、‘华农 7号’及‘河口龙牙’ 3个品种的胚性细胞悬浮系 。Meristematic globules were induced from immature mal e flowers of 7 different banana cultivars. The induction percentages of meristemat ic globules from immature male flowers was from 15% to 81%, depending on the cul tivars. Furthermore, both of the characters and the induction time for the appea rance of meristematic globules varied with the cultivars. Embryogenic cell suspe nsions (ECSs) of 3 banana cultuvars,‘Guangdong 2’, ‘Huanong 7’and‘Hekou Lo ngya’were established successfully from meristematic globules,and regener able plants were got successfully from the ECSs of the first two cultivars.

关 键 词：香蕉 分生小球体 分生途径 胚性细胞 悬浮系统 植株再生 

分 类 号：S668.1[农业科学—果树学] Q943.1[农业科学—园艺学]