梅单瓣复瓣花的相关分子标记初探  

Identification of RAPD and SCAR Markers Linked to the Single-double Flower Gene in Japanese Apricot(Prunus mume Sieb.et Zucc.)

作　　者：高志红[1] 韩振海[1] 章镇[2] 张玉明[2] 

机构地区：[1]中国农业大学园艺植物研究所,北京100094 [2]南京农业大学园艺学院,南京210095

出　　处：《园艺学报》2003年第5期612-614,共3页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(3 0 0 70 53 0 );国际基金资助项目(D/32 3 2 -1)

摘　　要：应用RAPD分子标记技术和SCAR分子标记技术研究了梅单瓣花与复瓣花相关的分子标记。结果表明从 34个随机引物中筛选到一个多态性引物OPP0 1,扩增出只有单瓣花类型具有的分子量为 1899bp的DNA特异片段 ,即OPP0 1 DS 1899;根据该序列设计大小分别为 2 5bp和 2 4bp的一对引物SSD 1和SSD 2 ,将RAPD标记转为SCAR标记 ,该标记大小为 10 79bp。Randomly amplified polymorphic DNA(RAPD)and sequence characterized amplified reg ion(SCAR)marker were performed to detect markers linked to the single double flo wer in Japanese apricot(Prunus mume Sieb.et Zucc.).Primer OPP01 was identif ied from 34 10-mer primers examined.The fragment was cloned and sequenced as 18 99 base pairs.According to the sequence,one primer pairs were designed to conver t the RAPD marker to SCAR marker.The length of the amplified fragment of SCAR ma rker was 1079 base pairs.

关 键 词：梅 单瓣花 复瓣花 分子标记 RAPD分子标记技术 SCAR分子标记技术 

分 类 号：S685.17[农业科学—观赏园艺]