超临界流体萃取法测定定虫隆残留  

Determination of the Residue of Chlorfluazuron with Supercritical Fluid Extraction

作　　者：杨立荣[1] 陈安良[1] 张兴[1] 

机构地区：[1]西北农林科技大学无公害农药研究服务中心

出　　处：《农药》2003年第11期26-28,共3页Agrochemicals

基　　金：948引进项目资助;项目编号 (9710 59)

摘　　要：将超临界流体萃取 (SupercriticalFluidExtraction ,SFE)技术与高效液相色谱分析相结合 ,得到了特异性杀虫剂定虫隆的残留分析方法。SFE对定虫隆的萃取条件为 :压力 3 0 0 0PSI ,温度75℃ ,静态萃取时间 2 0min ,动态萃取CO2 流量 50ml,改性剂 0 .0 8ml/g(甲醇 ) ,收集溶剂甲醇 ,萃取样品可直接用于高效液谱分析 ,条件为检测器 :紫外二极管阵列检测器 (UV2 54nm) ,色谱柱C18键合柱 ,流动相甲醇 /水 (80∶2 0 ,V/V) ,流速 1ml/min ,柱温 2 5℃ ,保留时间 :18.2 85min。该定虫隆残留分析方法回收率为 10 0 .94%。In this work we studied the optimum extraction conditions with supercritical fluid extraction(SFE) using 3factors and 5levels orthogonal design.Extracts were analyzed by HPLC with PAD.Many factors of the effects on SFE such as pressure,temperature,volume of dynamic extraction,time of static extraction,dose of modifier and trapping solvents etc.were investigated.The study proved that the optimum conditions to extract chlorfluazuron (a very polar pesticide) with SFE were as follow:pressure:3000PSI,temperature:75℃,time of static extraction:20min,volume of dynamic extraction:50ml,mdifier:0.08ml/g(matrix) methyl,trapping solvent:methyl.Under these conditions,the recovery of chlorfluazuron reached 100.94%.According to the experiments,we developed a simple and efficient method to extract and analyze residues from cabbage samples using SFE under moderate conditions.

关 键 词：超临界流体萃取 测定 定虫隆 农药残留 杀虫剂 

分 类 号：TQ450.7[化学工程—农药化工]