移植静脉粥样化与管壁蛋白聚糖关系的实验研究  

Relationship of atherogenesis and proteoglycans in wall of vein graft, an experimental study

作　　者：陈兵[1] 赵意平[1] 张柏根[1] 

机构地区：[1]上海第二医科大学附属仁济医院血管外科,200001

出　　处：《中华医学杂志》2003年第8期624-627,共4页National Medical Journal of China

基　　金：卫生部科研基金资助项目 ( 98- 2 - 2 89)

摘　　要：观察移植静脉粥样化过程 ,蛋白聚糖 (PG)变化同移植静脉粥样化关系。方法　将移植静脉蛋白聚糖对阴离子蛋白层析柱过柱 ,分为硫酸乙酰肝素蛋白聚糖 (HSPG)、硫酸软骨素蛋白聚糖(CSPG)及硫酸皮肤素蛋白聚糖 (DSPG) ,测定含量。结果　普食组术后 8周CSPG DSPG含量较正常静脉明显增加 (P <0 .0 5 ) ,术后 12～ 2 0周含量下降 ,接近正常 (P >0 .0 5 )。移植静脉术后 2 0周可见泡沫细胞形成 ;高脂组术后 8周CSPG DSPG含量较正常静脉明显增加 ,术后 12～ 2 0周持续在高水平 (P <0 .0 5 ) ,移植静脉术后 4周有泡沫细胞形成 ,术后 2 0周血管壁出现无组织结构区域。两组HSPG含量无明显区别 (P >0 .0 5 ) ,但其在总PG比例均降低。结论　CSPG DSPG对移植静脉粥样化具有促进作用 ,HSPG具有一定的保护作用。Objective To investigate the relationship between the concentrations of proteoglycans (PGs) and progress of atherogenesis in grafted vein. Methods A section of common carotid artery with a length of 0.5 cm was cut off and then a section of vein was implanted to the damage among 72 male New Zealand rabbits. Then the rabbits were randomly divided into two groups of 36 rabbits to be fed with high fat diet and ordinary diet respectively. Every six rabbits were killed one week, 2 weeks, 4 weeks, 8 weeks, 12 weeks, and 20 weeks after the operation. The implanted veins were taken to be observed by electron microscopy. The PGs in the implanted veins were extracted and divided into heparan sulfate proteoglycan (HSPG), chondroitin sulfate proteoglycan (CSPG), and dermatan sulfate proteoglycan (DSPG) by anion-exchange chromatography 8, 12, and 20 weeks postoperatively. The concentrations of PGs were measured. Blood was extracted from the heart to examine the level of LDL-cholesterol. The normal jugular veins on the opposite side were used as controls. Results The level of LDL-cholesterol in the high fat diet group was 26.5±7.49 mmol/L, significantly higher than that in the normal diet group (0.71±0.65 mmol/L, P<0.05). In the normal diet group, the content of CSPG-DSPG was 1 077±116 μg/g , significantly higher than that of the normal vein (809±75 μg/g, P<0.05) , and not significantly different from that of the normal diet group (1 077±116μg/g ) 8 weeks postoperatively, and became lower 12 and 20 weeks postoperatively (773±49 and 819±45 μg/g respectively) , both similar to that of normal vein (both P>0.05). Foam cells were found 20 weeks postoperatively. In the high fat diet group, the content of CSPG-DSPG was 1 089±94 μg/g 8 weeks postoperatively, significantly higher than that of normal vein (P<0.05), and the high level lasted till the 20 th postoperative week (1 068±100 μg/g , P<0.05). Foam cells were detected as early since the 4 th postoperative week, unorganized areas were found 20 weeks after

关 键 词：移植静脉粥样化 蛋白聚糖 实验研究 HSPG CSPG 

分 类 号：R654.3[医药卫生—外科学]