高产氢耐热菌株的分离鉴定及其放氢特性  

Isolation and Properties of a High Rate H_2-producing Thermoduric Bacteria Strain

作　　者：龙敏南[1] 龙传南[1] 邬小兵[1] 徐惠娟[1] 张凤章[1] 许良树[1] 

机构地区：[1]厦门大学生命科学学院微生物与海洋药物研究所,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2003年第6期687-690,共4页Journal of Xiamen University：Natural Science

基　　金：国家863项目(2001AA515040);国际科技合作重点项目(2002AA515030);国家自然科学基金(302703228)资助

摘　　要：从高温环境中分离得到具有高产氢活力的微生物菌株,经16SrRNA基因序列分析及生理生化特性分析,鉴定该菌株为Proteussp.该菌株能以葡萄糖、蔗糖、麦芽糖、乳糖和淀粉等多种有机物为底物催化产氢.该菌株利用葡萄糖产氢的最适条件为:葡萄糖浓度0.1mol/L;菌体细胞密度OD600=0.60;反应系统起始pH值7.0;反应温度40℃.在该条件下菌株的最高产氢活性可达8.05μmolH2/h·mgprot.A thermoduric bacteria strain with high H2producing activity was isolated from high temperature environment. It was identified to be Proteus sp. by the analysis of sequence of 16S rRNA gene and its physiological properties. The thermoduric bacteria Proteus sp. could use different organic substrates such as glucose, sucrose, maltose, lactose and soluble starch for H2production under fermentative conditions. And glucose was the favorite substrates for H2production. The optimum conditions for Proteus sp. H2production were: substrate concentration: 0.1 mol/L glucose; cell density: OD600=0.6; starting pH:7.0; reaction temperature:40℃. Under the optimum conditions, the maximum H2production activity reached 80.5 μmol H2/h·mg prot.The results suggest that Proteus sp. is an ideal material for biological H2production.

关 键 词：产氢菌株 分离 鉴定 放氢特性 制备 耐热菌株 氢能 生物制氢 

分 类 号：TK91[动力工程及工程热物理]