定量PCR法判定对虾白斑杆状病毒早期的感染和增殖  

An Early Time-course Study on the Infection and Proliferation of Shrimp White Spot Bacilliform Virus with Quantitative PCR

作　　者：兰永胜[1] 章晓波[2] 徐洵[2] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005 [2]国家海洋局第三海洋研究所,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2003年第6期777-780,共4页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金(30170728)资助

摘　　要：对虾白斑杆状病毒又称白斑综合症病毒,是对虾养殖业危害最严重的病原体,至今未找到有效的防治方法.充分了解病毒的分子生物学特性和分子致病机理,是病害防治的根本途径,了解病毒的动态增殖特征是该研究的基础.本文采用定量PCR技术,研究对虾白斑杆状病毒人工注射感染后,早期的增殖规律以及感染致死对虾的病毒累积.并对对虾感染病毒后存活时间与个体大小的关系进行了观察.研究发现初期感染病毒含量有短期下降,然后才呈现递增的过程.死亡对虾病毒累积量大于1011病毒粒子/毫克组织(P<0.01).而在4.6～11.6g范围内,感染对虾存活时间与对虾质量不存在相关性(P>0.2).Shrimp white spot bacilliform virus (WSBV) was the heaviest pathogen for shrimp culture. No efficient medicament had been founded. The acquisition of molecular characteristics of WSBV and its pathogenesis was most important for WSBV prevention. The research on dynamic proliferation of WSBV was the foundation of the study work. Quantitative PCR was applied to study the proliferation at early infection stage and accumulation of WSBV in died infected prawns. The relation between survival time and weight of prawns was also observed. After infection, the quantity of WSBV was declined before 4 h and increased gradually thereafter. The accumulation of WSBV was more than 1011virions/mg tissue in dead infected prawns. There was no relativity between survival time and weight of prawns ranging from 4.6 g to 11.6 g.

关 键 词：对虾白斑杆状病毒 感染 增殖 定量PCR 分子致病机理 

分 类 号：S945.4[农业科学—水产养殖]