牛胚胎干细胞建系研究进展及存在问题  

Progress and Problems on Establishing Bovine Embryonic Stem Cell Lines

作　　者：窦忠英[1] 徐小明[1] 华进联[1] 

机构地区：[1]国家干细胞工程技术研究分中心

出　　处：《农业生物技术学报》2003年第5期439-443,共5页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(30070374);陕西省重点项目;杨凌示范区科研专项资助。

摘　　要：牛胚胎干细胞具有重要的生物学意义和广阔的应用前景。回顾了牛胚胎干细胞的研究进展,阐述了牛胚胎干细胞生物学特征与表面抗原标志、建系方法和技术路线,系统分析了影响牛胚胎干细胞分离克隆的各种重要因素,并对目前建系中所面临的主要问题进行了探讨。Bovine embryonic stem (ES) cells have great significances in biology and widespread prospects in application. This paper reviewed the progress of bovine ES cells. Biological characteristics and markers of surface antigen, and several methods of establishing bovine ES cell lines were elaborated. Some important factors affected to isolate and clone bovine ES cells were analyzed systematically. Finally main problems and difficulties in establishing bovine ES cell lines were discussed.

关 键 词：牛 胚胎干细胞 胚胎生殖细胞 表面抗原 

分 类 号：S823[农业科学—畜牧学]