锌(Ⅱ)配合物与DNA作用的研究  

Study on the interaction between [Zn(phen)_2]^(2+) complex and DNA by the spectral methods

作　　者：胡瑞定[1] 朱伟琴[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《浙江师范大学学报（自然科学版）》2003年第4期379-383,共5页Journal of Zhejiang Normal University:Natural Sciences

摘　　要：采用紫外可见光谱、荧光光谱等光谱方法研究了配离子[Zn(phen)_2]^(2+)(phen=邻菲咯啉)与小牛胸腺DNA的相互作用。在DNA存在下,配合物的紫外吸收光谱产生了明显的减色效应。DNA的碱变性曲线在配合物的存在下向pH值增大的方向移动,增色效应减小。荧光光谱表明EB(溴化乙锭)-DNA体系的荧光强度随[Zn(phen))2]^(2+)的加入而迅速减弱,表明锌的邻菲咯啉配合物与DNA之间发生了插入作用。The interaction between [Zn(phen)2 ]2+ complex ion and DNA was investigated by absorption spectra,fluorescence spectra. Based on the measurement of A260 in the different pH medias,the alkaline denaturation curves of DNA was in the absence and presence of [Zn(phen)2]2+ complex ion obtained respectively. The results showed that the alkaline denaturation for [Zn(phen)2]2+ complex ion occurred at a higher pH than for DNA alone. The absorption of EB-DNA system in the presence of [Zn(phen)2]2+ showed that [Zn(phen)2]2+ could partly sustain the place of EB. The result of fluorescence spectra suggested that the [Zn(phen)2]2+ complex ion could make the fluorescence intensity of EB-DNA system decreased sharply. All the experimental results indicated that the intercalative binding mode was the major modal for the interaction of [Zn(phen)2]2+ complex ion with DNA.

关 键 词：锌配合物 DNA 紫外可见光谱 荧光光谱 插入作用 

分 类 号：Q523[生物学—生物化学] O614.241[理学—无机化学]