BALB/c突变无毛小鼠特异性免疫功能的研究  

Study on Specific Immune Function of BALB/c-Mutant Hairless Mice

作　　者：王冬平[1] 栾蓉晖[1] 李善如[1] 徐超斗[2] 李桂军[1] 

机构地区：[1]军事医学科学院实验动物中心,北京100071 [2]总后卫生部

出　　处：《中国实验动物学报》2003年第1期23-25,共3页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然基金课题 ( 39970 390 )

摘　　要：目的　BALB c突变无毛小鼠的皮肤和被毛结构的突变是否影响机体的免疫功能 ,为此研究其免疫功能。方法　通过流式细胞仪和ELISA方法。对特异性免疫指标CD4+ 、CD3+ 、CD8+ 、CD19+ 、IgG进行检测。结果　各项指标雌雄之间差异不显著 ,无毛小鼠的各项指标均低于其他表型的指标。各组之间方差分析结果 :CD8+ 差异显著 ,其他指标差异不显著。IgG抗体的吸光度的结果 ,三种表型之间差异不显著。结论　该小鼠的皮肤和被毛突变对免疫功能有一定的影响。Objective The mutant gene of BALB/c hairless mice affects skin and coat structure, but whether can it affect the immune function. Methods the parameters of CD4 +,CD3 +,CD8 +,CD19 +,IgG were tested by FCM and ELISA.Results 1.There were no significant differences in mice between sexes. 2. The parameters of the hairless mice were lower than those of other phenotypes. The parameter of CD8 + was significantly different by statistics. There were no significant differences among IgG of three phenotypes.Conclusion The mutant gene of BALB/c hairless mice affected the immune function.

关 键 词：小鼠 近交HRs 细胞 抗体生成 流式细胞仪 免疫功能 

分 类 号：R392.2[医药卫生—免疫学] Q959.837[医药卫生—基础医学]