三七皂甙Rg1改善D-半乳糖模型鼠学习记忆能力与其作用的可能因素  

Study of learning and memory function in D-galactose model mice improved by Sanchi Saponin Rg1 and its participating factors

作　　者：乔萍[1] 杨贵贞[1] 

机构地区：[1]吉林大学基础医学院,长春130021

出　　处：《中国免疫学杂志》2003年第11期772-774,共3页Chinese Journal of Immunology

摘　　要：目的 :探讨三七皂甙Rg1改善D 半乳糖模型鼠学习记忆能力与其作用的因素。方法 :皮下注射D 半乳糖建立衰老模型 ,并设三七皂甙Rg1给药组。采用跳台和Y 迷路法检测各实验组小鼠学习记忆能力 ,分光光度法检测脑细胞抗氧化能力 ,ELISA法检测IL 1β和IL 6含量 ,流式细胞术检测胞内钙指数及神经细胞凋亡百分数。 结果 :模型鼠脑细胞抗氧化能力降低 ,胞内钙超载且神经细胞凋亡 ,及炎症因子含量增加 ,与三七皂甙Rg1给药组比存在显著差异。结论 :三七皂甙Rg1通过抗氧化作用 ,降低氧化损伤所致脑海马区炎症因子含量增加 ,并减少神经细胞胞内钙超载和神经细胞凋亡。Objective:To investigate the role of sanchi Saponin Rg1 in D-galactose -induced amnesic mice ,and its possible mechanism.Methods:D-galactose was injected by subcutaneous to establish the aging model, and with drug treatment group.The Y-maze tests was performed to evaluate the learning and memory function, spectrophotometry was used to measure abilities of anti-oxidation.ELISA was adopted to evaluate the level of IL-1β and IL-6. Then FCM was applied to assay the index of intracellular calcium and the percentage of neural cell apoptosis.Results:Compared with drug treatment group, the model mice reduced significantly activites of anti -oxidation , the overload of intracelluer calcium and the increase in apoptosis of neural cells as well as the inflammation factor.Conclusion:Sanchi Saponin Rg1 decreased in the content of inflammation factor , the index of intracellular calcium and the percentage of apoptosis in neural cells , possibly by enchancing the antioxidant function.

关 键 词：D-半乳糖 胞内钙指数 凋亡 白细胞介素-6 白细胞介素-1Β 三七皂甙RG1 学习记忆能力 中药 药理学 

分 类 号：R285.5[医药卫生—中药学] R338.64[医药卫生—中医学]