微孔尼龙-6膜的催化水解改性和DNA芯片的原位合成  

Catalytic Hydrolysis and Modification of Microporous Nylon-6 Membranes and Insite Synthesis of DNA Chip Active Membranes

作　　者：汤建新[1] 刘正春[1] 李松[1] 聂立波[1] 肖鹏峰[1] 何农跃[1] 

机构地区：[1]东南大学教育部分子与生物分子电子学开放实验室,南京210096

出　　处：《高等学校化学学报》2003年第11期2089-2091,共3页Chemical Journal of Chinese Universities

基　　金：国家"八六三"计划 (批准号 :10 1-0 1-0 1);"九七三"计划 (批准号 :G19980 5 12 0 4);国家自然科学基金 (批准号 :60 0 710 0 1);江苏省高新技术项目 (批准号 :BG2 0 0 10 10 );教育部高等学校骨干教师资助计划基金资助

摘　　要：Microporous polyamide-6 membranes were hydrolyzed in 0.01 mol/L NaOH/CH 3OH medium by refluxing for about 36 h. By investigating the UV spectra, the hydrolyzing conditions were optimized. A pH value of 12 and reaction time of 36 h are the preferred conditions for hydrolysis. The treated membrane can be applied for in site synthesis of oligonucleotide and, for example, the oligonucleotide probes of 5′-AACCACCAAACACAC-3′ were successfully synthesized on the hydrolyzed membrane,which was proved by the high resolution X-ray photoelectron spectroscopy(XPS) of P 2p. The single step coupling efficiency was determined by UV spectra to be in excess of 98 %.Microporous polyamide-6 membranes were hydrolyzed in 0.01 mol/L NaOH/CH 3OH medium by refluxing for about 36 h. By investigating the UV spectra, the hydrolyzing conditions were optimized. A pH value of 12 and reaction time of 36 h are the preferred conditions for hydrolysis. The treated membrane can be applied for in site synthesis of oligonucleotide and, for example, the oligonucleotide probes of 5′-AACCACCAAACACAC-3′ were successfully synthesized on the hydrolyzed membrane,which was proved by the high resolution X-ray photoelectron spectroscopy(XPS) of P 2p. The single step coupling efficiency was determined by UV spectra to be in excess of 98 %.

关 键 词：尼龙-6膜 催化水解 改性 DNA芯片 原位合成 偶联效率 氨基 基因芯片 

分 类 号：Q75[生物学—分子生物学] TN4[电子电信—微电子学与固体电子学]