大鼠短间隔连续部分肝切除上调表达基因的克隆与分析  

Screening and Identification of Upregulated Expressed Genes by Suppression Subtractive Hybridization in Rat Liver Regeneration after Short Interval Successive Partial Hepatectomy

作　　者：李玉昌[1] 徐存拴 林俊堂[1] 张会勇[1] 李文强[1] 魏明旭[1] 张云汉[2] 

机构地区：[1]河南师范大学生命科学学院,新乡453002 [2]河南省肿瘤病理重点实验室,郑州450052

出　　处：《动物学杂志》2003年第1期22-27,共6页Chinese Journal of Zoology

基　　金：国家自然科学基金资助项目 (No.39970 36 2 ) ;河南省重大科技攻关项目 (No.0 12 2 0 31990 )

摘　　要：以短间隔连续部分肝切除 1 1 2h为试验方 ,以 0h对照为驱动方 ,应用抑制性消减杂交技术构建了高效率的正向消减cDNA文库 ,从中随机挑取的 5 0个克隆中有 45个包含了 1 0 0～ 3 5 0bp插入片段 ,对这些片段进行测序后经GenBankblast同源性检索 ,表明 8个片段均为未知新序列。In this study,suppression subtractive hybridiation(SSH) was carried out using normal rat liver tissue as the driver and the 112?h tissue following short interval successive partial hepatectomy(SISPH) as the tester to establish a highly efficient forward subtractive cDNA library.After scree\|ning,45 of 50 clones derived from the cDNA library were inserted into 100-450?bp cDNA fragments.Among these up regulated clones,8 cDNA fragments were from unknown sequences(GenBank accession number:BM403207,BM403208,BM403209,BM403210,BM285361,BM285365,BM285369,BM2853).

关 键 词：短间隔连续部分肝切除 抑制性消减杂交 肝再生 大鼠 上调表达基因 基因克隆 

分 类 号：Q786[生物学—分子生物学]