动物种群遗传多态性研究中的PCR技术  

The Application of the PCR Technique in the Study of Genetic Polymorphism in Animal Populations

作　　者：宋铭晶[1] 张知彬[1] 徐来祥[1] 

机构地区：[1]中国科学院动物研究所,北京100080

出　　处：《动物学杂志》2003年第1期93-97,共5页Chinese Journal of Zoology

基　　金：中国科学院重要创新方向 (No.KSCX2 1 0 3;KSCX2 SW 10 3) ;国家自然科学基金重点项目 (No.39730 0 90 )资助

摘　　要：基因组DNA的变异是种群遗传多态性研究的基础。PCR技术可以在反应管内经济快速地扩增特定DNA序列 ,在动物种群遗传多态性研究中的应用主要包括三个方面 :(1 )种群遗传多态位点的检测 ;(2 )基因定位或利用已经定位的单拷贝基因设计染色体位点特异的分子标记 ;(3 )与DNA测序技术相结合 。Genetic polymorphism of populations is based on variation in genomic DNA. The PCR technique can quickly and inexpensively amplify the specific DNA segments to ample quantities for further genetic analysis. Therefore, this technique is appropriate to the population genetics study. The application of this technique in population genetics mainly involves the following three aspects: (1) PCR based molecular markers were widely used to detect the genetic polymorphic loci of a population; (2) locate gene in the chromosome, or screen some kinds of individuals with sequence tagged sites markers;(3)the combination of PCR amplification and DNA sequencing make it easy to obtain the nucleotide sequences of some specific genes.

关 键 词：PCR技术 动物种群 遗传多态性 分子标记 基因变异 基因定位 DNA测序 

分 类 号：Q953[生物学—动物学]