对硝基苯基α-D-葡萄糖苷的合成及应用  

Synthesis of P-Nitrophenyl-α-D-Glucopyranoside and Its Application

作　　者：徐汝明[1] 张建华[1] 陆阳[1] 

机构地区：[1]上海第二医科大学化学教研室,上海200025

出　　处：《上海第二医科大学学报》2003年第6期489-491,共3页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：上海市教委资助项目(2000QN89)

摘　　要：目的合成α-D-葡萄糖苷酶检测底物对硝基苯基α-D-葡萄糖苷,建立临床检测方法。 方法采用Westphal-Feier合成法制备对硝基苯基α-D-葡萄糖苷;依据α-葡萄糖苷酶作用于底物的动力学性质,建立α-葡萄糖苷酶的检验方法。 结果获得高纯度底物对硝基苯基α-D-葡萄糖苷,纯度>99.0％,并开发成试剂盒,批内变异<2.5％,批间变异<3.8％,阳检率85.7％。 结论试剂盒符合临床应用要求。Objective To synthesize p - nitrophenyl -α-D-glucopyranoside as a substrate for α- glucosidase, and develope a method for testing its activity. Methods The substrate was prepared by Westphal and Feier synthesis. A quantitative method was established according to α-glucosidase' s kinetic characters on the substrate. Results A highly purified substrate (purity >99.0% ) was obtained. A kit for α-glucosidase was made with OCV < 2. 5% , RCV < 3. 8% , and sensitivity 85. 7% . Conclusion The kit can be reliably used in clinics.

关 键 词：硝基苯基α-D-葡萄糖苷 合成 动力学性质 男性不育 

分 类 号：O629.25[理学—有机化学]