芹灵冲剂的急性和长期毒性实验研究  

STUDY ON ACUTE AND LONG-TERM TOXICITY OF QINLING CHONGJI

作　　者：杨新波[1] 黄正明[1] 曹文斌[1] 陈鸿雁[1] 郑鸣[1] 张敬珍[1] 李树林[2] 刘军[3] 刘琳[3] 

机构地区：[1]中国人民解放军北京高等医学专科学校药理教研室,北京100071 [2]中国人民解放军第309医院病理科,北京100090 [3]中国人民解放军第307医院生化检验科,北京100083

出　　处：《解放军药学学报》1999年第3期11-14,共4页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金："九五"规划军队重点科研基金

摘　　要：目的：观察芹灵冲剂对动物的急性、长期毒性及中毒靶器官，为临床安全用药提供依据。方法：急性毒性实验用小鼠以口服和腹腔给药，分别测定ＬＤ５０ 和ＭＤＴ，并推算出相当临床人用量的倍数。长期毒性实验用大白鼠，设３０ｇ·ｋｇ－１ 、１０ｇ·ｋｇ－１ 两个剂量组和生理盐水对照组，连续灌服９０ｄ，观察动物的饮食、体重。于实验第４５ｄ、９０ｄ 和停药后２８ｄ，分别各处死１／３动物，取血测定血常规及其生化指标，并取出主要脏器作组织病理学检查。结果：芹灵冲剂的口服ＬＤ５０ 未能测出，ＭＴＤ为１００ｇ·ｋｇ－１ ，相当于人临床用量的２００ 倍。注射剂腹腔注射ＬＤ５０ 为１９．７３ｇ·ｋｇ－１ 。芹灵冲剂慢性毒性实验，大鼠连续灌服９０ｄ，小剂量１０ｇ·ｋｇ－１ 和大剂量３０ｇ·ｋｇ－１ 组动物饮食、体重正常，血液及生化指标均未见异常，主要脏器组织学观察未见病理改变。结论：芹灵冲剂口服用药。AIM The acute and long-term toxicity of Qinling Chongji(QL) and toxical target organ in animals,were observed to provide the basis for the safty of using this drug in clinical.METHODS Mice were used in acute toxicity experiments. Oral LD 50 and maximal tolerance dose (MTD) were determined,and the doses for human being were calculated accordingly. Long-term toxicity was performed in rats, two treatment groups( QL 30g·kg -1 and QL 10g·kg -1 ) and control group(equal volume of NS) were divided up. QL was administered orally for 90 days.The effects of QL on blood routine examination, biochemical blood test and main organs' pathology were investigated.RESULTS Oral LD 50 of QL could not be obtained, while intraperitoneal LD 50 was 19.73g·kg -1 .MTD was 100g·kg -1 ,equal to 200 times dosage in human.In long-term toxicity experiment, the animals in both low and high doses(10g·kg -1 or 30g·kg -1 ) of QL groups had normal diets ,and no changes were observed in blood routine examination,biochemical blood test and pathology after administration for 90 days.CONCLUTION The present study show that it is safty to take QL for oral administration.

关 键 词：芹灵冲剂 长期毒性 急性中毒 实验 病理学 

分 类 号：R965[医药卫生—药理学]