十全大补丸防护环孢菌素A肾毒性的实验研究  

PROTECTIVE EFFECTS OF SHIQUANDABU BOLUS ON KIDNEITOXICITY INDUCED CYCLOSPORINL IN RATS

作　　者：张黎明[1] 朱丽青[1] 吴秀华[2] 

机构地区：[1]中国人民解放军济南军区总医院药理科,济南250031 [2]中国人民解放军106医院,济南250022

出　　处：《解放军药学学报》1999年第3期17-20,共4页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的：以急性环孢菌素Ａ（ＣｓＡ） 肾毒性大鼠为模型，观察气血双补药十全大补丸防护ＣｓＡ 肾毒性的作用。方法：大鼠分为４ 组：ⅠＣｓＡ组（０．０５ｇ·ｋｇ－１） ，ⅡＣｓＡ加十全大补丸高剂量组（０．０５ｇ·ｋｇ－１ ＋ ０．６ｇ·ｋｇ－１） ，ⅢＣｓＡ加十全大补丸低剂量组（０．０５ｇ·ｋｇ－１ ＋ ０．３ｇ·ｋｇ－ １） ，Ⅳ空白对照组（ 等量２％ 羧甲基纤维素ＣＭＣ） ｑｄ，ｉｇ，连续７ｄ。末次给药后，单个收集大鼠２４ｈ 尿液；末次给药２４ｈ 后，眼眶取血，测定超氧化物歧化酶（ＳＯＤ）、丙二醛（ＭＤＡ） 、谷胱甘肽ｓ－ 转移酶（ＧＳＴ） 、肌酐（Ｃｒ） 、尿素氮（ＢＵＮ） 。取肾脏作病理检查。结果：用药７ｄ 后，ＣｓＡ组２４ｈ 尿量明显减少，十全大补丸组明显增加（ Ｐ＜ ０．０１）；ＣｓＡ组Ｃｒ、ＢＵＮ上升，十全大补丸两组降低（ Ｐ＜０ ．０５） ；各组ＳＯＤ、ＧＳＴ活力无显著性差异，十全大补丸高低剂量组ＭＤＡ 较ＣｓＡ 组明显升高（ Ｐ＜ ０．０１） 。结论：十全大补丸对ＣｓＡ 所致大鼠肾毒性有保护作用。AIM To observ the protective effects of Shiquandabu bolus(SQDB) on kidneitoxicity induced cyclosporine(CsA) in rats.METHODS Acute Kidneitoxicity Wistar rats were dividcd into the four groups,CsA(0.05g·kg -1 ),CsA(0.05g·kg -1 )+SQDB(0.6g·kg -1 ),CsA(0.05g·kg -1 )+SQDB(0.3g·kg -1 )and normal saline(2%CMC-Na) be given separatly (ig,qd) for seven days. After the last dose of the drugs,the urine was collected for 24 hours. After 24 hours of the last dose of the drugs,superoxide dismutase(SOD),malondialdehyde(MDA), glutathione s-trans -ferase(GST), creatine(Cr) and blood urea nitrogen(BUN) in serum were determined and the kidney tissue was observed with the pathological method.RESULTS After seven days,in group SQDB of both,urine volume was increased significantly but in group CsA was decreaed. The results of Cr and BUN were contrary to the above temonstrotion.There were no significant changes in all groups for SOD and GST.The levels of MDA in two SQDB groups were higher but in the CsA group lower than those in the normal.CONCLUSION SQDB has the effects of increasing kidny function but no effect of anti-oxidation and removing free radical.

关 键 词：十全大补丸 环孢菌素A 肾毒性 CSA 免疫抑制剂 

分 类 号：R965[医药卫生—药理学]