薄层扫描法测定复方苦参注射液中苦参碱的含量  

DETERMINATION OF MATRINE IN COMPOUND RADIX SOPHORAE FLAVESCENTIS INJECTION BY TLCS

作　　者：宋茹[1] 张晓席[1] 袁继民[1] 于连生[1] 邓春光[1] 

机构地区：[1]中国人民解放军济南军区药品检验所,济南250022

出　　处：《解放军药学学报》1999年第6期43-44,50,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的 :用薄层扫描法对复方苦参注射液中苦参碱进行了含量测定。方法 :以甲苯 -丙酮 -乙醇 -氨水 ( 10∶10∶1∶0 .1)为展开剂 ,在CS - 90 0 0飞点薄层扫描仪上以反射式锯齿扫描方式进行测定 ,λ =5 15nm。结果 :样品含量在 2 .16～ 7.5 6μg范围内呈良好线性关系 ,r =0 .9992 (n =6) ,平均回收率 99.0 % ,RSD =0 .61%。结论 :本法简便、快速、精确 ,重现性好 ,可作为复方苦参注射液的质量控制标准。AIM A method of the quantitative determination of matrine in compound radix sophorae flavescentis injection by TLCS.METHODS The mobile phase was toluene:acetone:ethanol:ammonia water(10∶10∶1∶0.1).The scanning method was dual wavelegth and zigzag with CS-9000 scanner.The detection wavelength was 515nm.RESULTS It showed a good linearity between 2.16-7.56μg with the correlation coefficient 0.999 2.The average recovery was 99.0%,RSD=0.61%.CONCLUSIONS The method is easy,rapid,accurate and reproducible.It can be a quality control standard of compound radix sophorae flavescentis injection.

关 键 词：薄层扫描法 复方苦参注射液 苦参碱 药物实验 天然药材 

分 类 号：R286[医药卫生—中药学]