Production of D-p-Hydroxyphenylglycine from DL-5-p-Hydroxyphenyl Hydantoin by Immobilized Pseudomonas Putida Cells in a Hollow Fiber Membrane Bioreactor  

作　　者：张铭俊 张衍坤 李京华 李曙光 虞星炬 

出　　处：《Chinese Journal of Chemical Engineering》1999年第1期86-90,共5页中国化学工程学报（英文版）

摘　　要：An immobilized cell membrane bioreaction system was developed to promote cell stability. The hollow fiber membrane bioreactor with immobilized Pseudomonas putida cells, operating in continual repeated batch operation mode was used for producing D-p-hydroxyphenylglycine from DL-5-p-hydroxyphenyl hydantoin. The concentration of N-carbamyl-D-p-hydroxyphenylglycine and D-p-hydroxyphenylglycine in the efflux was analyzed by high performance liquid chromatography at different intervals.1 INTRODUCTIOND-p-Hydroxyphenylglycine(D-pHPG) is one of the valuable intermediates in the production ofsemisynthetic β-lactam antibiotics.It can be used for the production of semisynthetic penicillinand cephalosporin.With the increasing demand for semisynthetic antibiotics,the productionof D-pHPG from DL-5-P-hydroxyphenyl hydantoin(DL-pHPH)has received more and more at-tention.Compared with the chemo-enzymatic route developed by Yamada et al.,the

关 键 词：D-P-HYDROXYPHENYLGLYCINE immobilized Pseudomonas putida cells hollow fiber mem-brane bioreactor 

分 类 号：TQ465[化学工程—制药化工]