17β-雌二醇促进鲻鱼性腺发育的作用机制:雌激素受体的定位  

Action mechanisms of estradiol-17βto promote gonadal development of grey mullet:localization of estrogen receptors

作　　者：方永强[1] 张为民[2] 翁幼竹[1] 

机构地区：[1]国家海洋局第三海洋研究所,福建厦门361005 [2]中山大学水生经济动物研究所,广东广州510275

出　　处：《台湾海峡》2003年第4期422-425,共4页Journal of Oceanography In Taiwan Strait

基　　金：福建省重中之重资助项目;国家自然科学基金倾斜项目(30070598)

摘　　要：应用免疫组织化学技术对雌激素受体α和β亚型在鲻鱼性腺进行定位研究.结果显示,在早期卵巢,α与β受体蛋白均在早期初级卵母细胞的卵被膜、核膜和核仁表达,受体阳性物质沿胞膜分布,核显免疫阴性;在卵黄发生期,ER-α和β则在卵被膜、卵黄膜和核膜表达.在精巢,α与β受体蛋白在生精细管生殖被膜、精原细胞、初级和次级精母细胞、精子细胞、足细胞和间质细胞表达,受体免疫阳性物质沿细胞膜分布.这些结果表明,鲻鱼性腺(卵巢和精巢)存在雌激素α与β受体亚型,该受体在介导17β-雌二醇对性腺的生理效应中起重要作用.Estrogen receptor a and β subtypes in the gonads (ovary and testis) of grey mullet were localized by using immunohistochemical technique. The results showed that in the early ovary a and β receptor protein were expressed in the egg envelope and nuclear membrane of early primary oocytes. The positive substance of receptor distributed along cellular membrane, and nucleus showed immuno-negative reaction. In the vitellogenetic stage, ER-a and β were expressed in the egg envelope, yolky membrane and nuclear membrane of oocytes. In testis, a and β receptor protein were expressed in the reproductive envelope of testis, spermatogonia, primary and secondary spermatocytes, spermatotids, Sertoli cell and Leydig cell, in which immuno-positive substance of receptor distributed along cellular membrane. These results indicated that estrogen receptor-a and β subtypes existed in the gonads (ovary and testis) of grey mullet and play an important role in mediating the physiological effects of estradiol-17β on gonds.

关 键 词：生殖生物学 免疫组织化学 雌激素受体 17Β-雌二醇 鲻鱼 性腺 作用机制 

分 类 号：Q25[生物学—细胞生物学] S917.4[农业科学—水产科学]