焦化废水处理系统微生物群落结构动态的ERIC-PCR指纹图谱分析  

Analysis of the microbial community of activated sludge in different aeration basins within an industrial phenol remediation system by ERIC-PCR fingerprinting

作　　者：高平平[1,2] 赵义[3] 赵立平[2] 

机构地区：[1]山西大学生物技术研究所,太原030006 [2]上海交通大学生命科学技术学院,微生物分子生态学与基因组学实验室,上海200240 [3]太原理工大学环境工程系,太原030024

出　　处：《环境科学学报》2003年第6期705-710,共6页Acta Scientiae Circumstantiae

基　　金：国家高科技研究发展计划项目 ("86 3"项目计划 ) (No .SZ 0 3 0 1 0 4;2 0 0 1AA2 14 131)

摘　　要：ERIC PCR群落指纹图谱监测与常规技术相结合 ,分析了焦化废水处理系统曝气池的微生物群落结构动态变化与污染负荷和主要污染物降解效果变化的关系 .在每 3d 1次 ,连续 8个时间点的监测中 ,待处理废水中苯酚负荷在 35 5 3mg L和132 82mg L之间波动 ,氰化物负荷从 0 96mg L变化到 34 75mg L ,CODCr最低为 10 4 4 10mg L ,最高时为 7930 90mg L .第 4监测期间高浓度矿物油冲击使活性污泥的沉降能力和对CODCr的降解效率显著降低 ,但对苯酚和氰化物的降解能力造成的影响较小 .监测期间 2个曝气池微生物群落的ERIC PCR指纹图谱的平均相似性系数 (Cs)分别为 94 1%和 96 6 % ,多样性指数在 1 77— 2 34和 1 6 0— 2 16之间 ,表明用ERIC PCR检测出的该系统中的活性污泥这部分微生物种群的结构比较稳定 ,可能构成了苯酚和氰化物去除效率在监测期间相对稳定的基础 .The occurrence, settleability and activity of activated sludge in first (TJ1) and second (TJ2) aeration basin in a AB process based industrial phenol remediation system were analyzed by ERIC-PCR(Enterobacterial Repetitive Intergenic Consensus-PCR) fingerprinting combined with activated sludge characterization (SV_(30)、MLSS、SVI), and major pollutants (phenol, cyanide and COD_(Cr)) measurements. During the monitoring period (one sampling every three days for 8 continuous samplings, M1—M8), the concentrations of primary pollutants in the influent fluctuated from 35.53mg/L to 132.82mg/L for phenol, between 0.96mg/L to 34.75mg/L for cyanide and from 1044.10mg/L to 7930.90mg/L for COD_(Cr). The presence of a pollution shock caused by mineral oil contamination occurred at M4 monitoring stage resulted in a dramatic decrease in activated sludge settleability and COD_(Cr) removal efficiency. The introduction of this loading shock had no significant impact on the system's ability to degrade phenol and cyanide. ERIC-PCR banding patterns of activated sludge samples at 8 stages showed a mean C_s value of 94.1% within TJ1 and 96.6% within TJ2, Shannon-Weaver indices of 1.77—2.34 in TJ1 and 1.60—2.16 in TJ2. Populations in the system detected by ERIC-PCR fingerprinting were thus fairly stable. The stability of the ERIC-PCR amplifiable populations in activated sludge may serve as the basis for relatively stable removal efficiency of phenol and cyanide during the monitoring period.

关 键 词：焦化废水 水处理系统 微生物群落结构 活性污泥 ERIC-PCR指纹图谱 生物监测 

分 类 号：X784.03[环境科学与工程—环境工程] X835