二氧化硫对大鼠肺泡巨噬细胞DNA的损伤作用  

Damage effects of sulfur dioxide inhalation on DNA damages in alveolar macrophages from male rats

作　　者：孟紫强[1] 白巨利[1] 

机构地区：[1]山西大学环境医学与毒理学研究所,太原030006

出　　处：《环境科学学报》2003年第6期825-827,共3页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金资助项目 (No .3 0 0 70 647) ;山西省自然科学基金资助项目

摘　　要：运用单细胞凝胶电泳技术 (又称彗星实验 )研究了SO2 动式吸入对雄性Wistar大鼠肺泡巨噬细胞DNA的损伤作用 .结果表明 ,在SO2 浓度为 2 8 6,57 3 ,114 4mg m3 的动式吸入染毒条件下 ,雄性大鼠肺泡巨噬细胞DNA拖尾长度分别为 7 59,12 3 9,2 1 2 2 μm ,表明SO2 可引起大鼠肺泡巨噬细胞DNA损伤 ,且随SO2 吸入浓度的增加而加剧 ,呈明确的剂量效应关系 .这意味着SO2 具有引起哺乳动物肺泡巨噬细胞DNA突变的潜在危险 。The effects of SO_2 dynamic inhalation on DNA damages in alveolar macrophages of male rats were studied with the single cell electrophoresis technique(Comet assay).The results showed that after exposure to SO_2 at 28.6,57.3,114.4mg/m+3,tail lengths of nuclear DNA in the alveolar macrophages from male rats were 7.59,12.39,21.22μm,respectively.SO_2 could lead to DNA damages in alveolar macrophages in a dose-dependent manner.It implies that SO_2 has a potential risk to cause DNA mutation of alveolar macrophages from mammalian animals,and it may cause damages to the engulf function and anti-tumor function of alveolar macrophages.=

关 键 词：二氧化硫 肺泡巨噬细胞 DNA损伤 肺泡巨噬细胞 彗星实验 大鼠 

分 类 号：R994.6[医药卫生—毒理学]