正常及肿瘤染色质的荧光寿命法、精确量热法和荧光能量转换  

Study on the Wistar Rat Liver and Its Walker Tumor Chromatins by Fluorescence Lifetime and Calorimetric Measurements

作　　者：祁超[1] 赵剑非[1] 李伟国[2] 杜丽[3] 刘立岩[2] 

机构地区：[1]中国科学技术大学生命科学学院结构生物学重点实验室,合肥230027 [2]中国科学院长春应用化学研究所稀土化学与物理重点实验室 [3]中国人民解放军军需大学生物化学教研室,长春130062

出　　处：《应用化学》2003年第12期1135-1138,共4页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金 ( 2 970 10 0 5 );中国科学院基础局重大项目 (KJ95 1 A1 5 0 4 0 2 )资助项目

摘　　要：通过DNA染色剂 染色质配合过程的荧光寿命、精确量热和荧光能量转换的测定 ,研究了正常染色质 (NC)和肿瘤染色质 (TC)的结构。结果表明 ,NC中溴化乙锭插入DNA的成分比TC增加了 4 86 % ;溴化乙锭与TC中DNA的配合焓为 - 15 38kJ/mol,NC中DNA的配合焓为 - 13 2 5kJ/mol;TC中丹磺酰氯和吖啶橙之间的荧光能量转换效率比NC中的低 ,而平均供体 受体距离比NC的大 ,说明TC刚性结构较少、与溴化乙锭结合的可能性较大及基因活性较强。The structures of rat liver chrotin and its walker tumoral chromatin have been studied by fluorescence lifetime, precision calorimetric determinations and energy transfer measurement. The results show that the component with ethidium bromide inserting DNA in tumoral chromatin(TC) increases by 4.86% than that in normal chromatin(NC). The formation enthalpy of the complex from ethidium bromide and DNA in TC is -15.38 kJ/mol and -13.25 kJ/mol for NC. The energy transfer efficiency between dansyl chloride and acridine orange in labeled TC is lower than that in labeled NC. The mean donor-acceptor distance is longer than that in labeled NC. These results denote that TC has a less rigid structure and higher genic activity, and can more easily form complex with ethidium bromide.

关 键 词：量热学 染色质 能量转换 荧光寿命 

分 类 号：O629[理学—有机化学]