烟草抗CMV突变体的抗病性分析  

Analysis of the tobacco mutants resistant to CMV

作　　者：王万军[1] 曹建军[2] 王文芳[2] 阎福秀[1] 

机构地区：[1]西南交通大学生物工程系,成都610031 [2]西北农林科技大学生命科学学院,陕西杨陵712100

出　　处：《西北植物学报》2003年第10期1776-1779,共4页Acta Botanica Boreali-Occidentalia Sinica

摘　　要：在突变体植株 Ea201主、Ea201侧、Aa201侧 的5～6叶期接种CMV汁液,并按优选法去除病症最严重的病株,6周后将剩余植株分别移栽到田间露地和防虫网内,到盛花期分别统计植株的感病情况.其结果为:在田间露地和防虫网内,1感病的对照品种的植株高度只及健康的对照植株高度的30%和40%,突变体的则可达到70%和80%;2对照品种的植株感CMV的病情指数可达90%和50%,突变体的则只有30%和3%;3对照品种植株感TMV的病情指数可达60%和30%,突变体植株感TMV的病情指数只有30%和10%;4突变体植株群体中有症状回复现象,同时其育性不受任何影响.从而表明所筛选到的烟草突变体 Ea201主、Ea201侧、Aa201侧 对CMV、TMV的相对抗性较好,具有较高的抗病性.The younger leaves of tobacco M2 mutants resistant to CMV were inoculated with poisonous fluid of CMV at the stage of 5~6 leaves.38% of the most serious infected plants were pulled out per week.The residual plants were respectively transplanted in the field and in the room of the nylon net after 6 weeks.At the flowering stage,the height of the infected control plants in the field and in the room of the nylon net were about 30% and 40% to the healthy plants,while height of the infected mutant plants were about 70% and 80% to the healthy plants.The disease index to CMV of the infected control plants in the field and in the nylon net were about 90% and 50%,while disease index to CMV of the infected mutant plants were about 30% and 3%.The disease index to TMV of the infected control plants in the field and in the nylon net were about 60% and 30%,while disease index to TMV of the infected mutant plants were about 30% and 10%.There was a phenomena in the population of the mutant plants that symptom of the disease was reinstated to health and the flowering and fertilization was not affected.These indicated that the resistance to CMV and TMV of the tobacco mutants,Ea 201 main,Ea 201 side and Aa 201 side,is higher.

关 键 词：烟草 抗CMV突变体 生长势 病情指数 

分 类 号：Q946-33[生物学—植物学] S572[农业科学—烟草工业]