利用乙酰胆碱酯酶传感器监测海水久效磷  

Detection of monocrotopphos in seawater using an biosensor based on acetylcholinesterase

作　　者：孟范平[1] 唐学玺[1] 李桂芳[1] 曹立民[1] 李永祺[1] 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《海洋环境科学》2003年第4期63-67,共5页Marine Environmental Science

基　　金：十五"国家863计划(2001AA635130)

摘　　要：用戊二醛(GA)将乙酰胆碱酯酶(AChE)交联在透析膜上,并与pH电极结合,由此开发出一种基于固定化酶的电位型传感器。该酶电极可以快速、灵敏地测定海水中的久效磷。仪器对海水中10-10～10-7的久效磷农药具有极好的线性响应,检测下限为0.1μg／L,加标回收率在90%～103%之间,分析1个海水样品只需50min。In this paper, a potentiometric biosensor based on immobilized enzyme was developed by crosslinking acetylcholinesterase (AChE) with glutaraldehyde on a piece of dialytic membrane at the pH electrode surface. The AChE-electrode was used for the (rapid) and sensitive detection of monocrotophos in seawater. It showed a better linear response to monocrotophos in seawater when its concentration changed from 10^(-10) to 10^(-7). In additon, it had a detection limit of 0.1μg/L and a recovery rate from 90% to 103%. With this biosensor, detecting one sample could finish in 50 minutes.

关 键 词：乙酰胆碱醇酶(AChE) 酶电极 生物传感器 海水 久效磷 

分 类 号：X8[环境科学与工程—环境工程]