黄胸散白蚁纤维素酶的研究  

Digestive cellulase from the damp-wood termite Reticulitermes flaviceps(Oshima)(Rhinotermitidae:Reticulitermes)

作　　者：杨天赐[1] 莫建初[1] 程家安[1] 刘文军 

机构地区：[1]浙江大学城市昆虫学研究中心,浙江杭州310029 [2]宁波市白蚁防治所,浙江宁波315010

出　　处：《中国森林病虫》2003年第6期4-7,共4页Forest Pest and Disease

基　　金：教育部留学归国人员科研启动基金;浙江大学留学归国人员科研启动基金资助项目。

摘　　要：对黄胸散白蚁体内纤维素酶所做的研究结果表明 ,37℃时 ,C1酶与pNPC底物反应的最佳时间为 1h ,Cx 酶与CMC Na和 β 葡糖苷酶与Salicin反应的最佳时间均为 0 2 5h。黄胸散白蚁工蚁头部纤维素酶主要是Cx 酶和 β 葡糖苷酶。肠道组织中 ,3种纤维素酶主要分布于前肠和后肠组织。Cx 酶在前肠的酶量占肠道组织该酶总量的 5 2 9% ;C1和 β 葡糖苷酶绝大部分在后肠 ,在该部位所含酶量分别占整个肠道组织酶总量的 83 3%和 80 2 %。说明The digestive cellulase from Reticulitermes flaviceps was determined in this study.The results indicated that when the cellobiohydrolase reacted with p-nitrophenyl-β-D-cellobioside at 37 ℃,the activity of enzyme reached the maximum at 1 hour after reaction.For endo-β-14-glucanase and β-glucosidase,the best reactive time was 0.25 hour when they reacted with CMC-Na and salicin separately.In the head of R.flaviceps (worker,)the endo-1,4-β-glucanase and β-glucosidase were main cellulases.As for the whole gut tissues,cellulases mainly distributed in the foregut and hindgut.Endo-β-14-glucanase in the foregut accounted for 52.9% of cellulases amount in the whole gut tissues.Cellobiohydrolase and β-glucosidase mostly located at the hindgut,at which they accounted for 83.3% and 80.2% of the total cellulases in gut tissue respectively.These results showed that the activities of cellulase varied in the different tissues in R. flaviceps.

关 键 词：黄胸散白蚁 内切-β-1 4-葡聚糖酶 纤维二糖水解酶 Β-葡糖苷酶 

分 类 号：S763.33[农业科学—森林保护学]