严重烧伤后小鼠脾脏T淋巴细胞跨膜信号转导的改变与IL-2、IL-10分泌  

The postburn change in splenic T lymphocyte transmembrane signal transduction and its relationship with the secretion of IL-2 and IL-10 in severely scalded mice.

作　　者：郑峻松[1] 吴军[1] 彭代智[1] 黄文华[1] 肖光夏[1] 黎鳌[1] 罗中华[1] 

机构地区：[1]第三军医大学西南医院烧伤研究所,重庆400038

出　　处：《中华烧伤杂志》2000年第6期352-354,共3页Chinese Journal of Burns

基　　金：国家自然科学基金资助项目 !(395 0 0 14 9)

摘　　要：目的　探索严重烧伤后小鼠脾脏T淋巴细胞功能改变及IL - 2、IL - 10分泌规律 ,并从T淋巴细胞活化跨膜信号转导途径寻找导致其改变的原因。　方法　检测T淋巴细胞表面抗原受体TCRα/ β ,辅助刺激分子CD2 8及参与跨膜信号转导的G蛋白、蛋白酪氨酸激酶 (PTK)、蛋白激酶C(PKC)的活性改变 ,观察伤后不同时相T淋巴细胞增殖转化功能及IL - 2、IL - 10分泌活性 ,分析各信号转导分子改变与T淋巴细胞功能活性改变之间的关系。　结果　烧伤后T细胞膜表面分子TCRα/β、CD2 8表达阳性率降低 ,GTPase活性和膜PTK活性均受抑 ,但于伤后 16 8h恢复 ,转膜PKC活性出现先降低后升高的双相改变 ,且与IL - 10水平密切相关。结论　烧伤后T细胞膜表面分子TCRα/ β、CD2 8和跨膜信号转导酶活性的改变是导致伤后IL - 2分泌降低、T细胞功能受抑和IL -Objective ToexplorethepostburnfunctionalchangeinsplenicTlymhocytesandthesecretory rulesofIL - 2andIL - 10inseverelyscaldedmice ,andtolookfortheexplanationofthechangebymeansofT lymphocytetransmembranesignaltransdnctionstudy . Methods ThechangesintheantigenreceptorsonTcells (TCRα/β) ,assistingstimulatingmolecule(CD2 8)andtheactivitiesofGTPase ,proteintyrosinekinase(PTK)and proteinkinaseC (PKC)thatparticipatedinthetransmembranesignaltransductionweredetected ,soastoobserve theproliferationandtransformationfunctionofTcellsatdifferentpostburnperiodsandthesecretionofIL - 2and IL - 10 .Furthermore ,thechangesineverysignaltransductionmoleculeswereanalyzedwiththeconsiderationof theirrelationshipwiththechangeinTcellfunctionactivities. Results Thepostburnpositiveratesofthe expressionsofTCRα/βandCD2 8onTcellmembranedecreased .ThepostburnGTPaseactivityandmembranePTK activitywereallsuppressedbutrecoveredat16 8postburnhours .MembranePKCactivityexhibitedadual -phase change (increasefollowingdecrease) ,whichwascloselyrelatedtoIL - 10level. Conclusion Thepostburn changesinTCRα/β,CD2 8onTcellmembranemoleculesandtransmembranesignaltransductionenzymeswere importantfactorscontributingtothedecreaseinIL - 2secretion ,suppressedTcellfunctionandthedual - directionalchangesinIL - 10secretion .

关 键 词：严重烧伤 脾脏 T淋巴细胞 跨膜信号转导 白细胞介素-2 白细胞介素-10 

分 类 号：R644[医药卫生—外科学]