Sequence analysis and functional study of the Han Nationality glial cell line-derived neurotrophic factor transcript  

作　　者：陈哲宇 黄爱军 路长林 吴祥甫 何成 

机构地区：[1]Department of Neurobiology,Department of Basic Medicine,Second Military Medical University,Shanghai 200433,China [2]Shanghai Institute of Biochemistry,Chinese Academy of Sciences,Shanghai 200433,China

出　　处：《Journal of Medical Colleges of PLA(China)》2001年第1期55-59,共5页中国人民解放军军医大学学报（英文版）

基　　金：National Natural Science Foundation of China (No.30000048) ; National Basic Research Program of China (G1999054000)

摘　　要：Objective: To study the sequence and function of the glial cell line-derived neurotrophic factor (GDNF) transcript in subjects of Han nationality. Methods: The Han nationality GDNF transcript was amplified by RT-PCR and expressed by baculovirus expression system. Biological activity of the expressed product was measured by the primary culture of midbrain dopaminergic neurons. Results: There only existed the shorter GDNF transcript of 555 bp in the Han nationality. The secretory expression product of the shorter transcript in insect cells promoted the survival and differentiation of dopaminergic neurons. Conclusion: It is found that there is a 78 bp deletion in the Han nationality GDNF transcript compared with the reported 633 bp GDNF transcript. The 78 bp deletion does not affect the secretory expression and biological activity of GDNF mature protein.Objective: To study the sequence and function of the glial cell line-derived neurotrophic factor (GDNF) transcript in subjects of Han nationality. Methods: The Han nationality GDNF transcript was amplified by RT-PCR and expressed by baculovirus expression system. Biological activity of the expressed product was measured by the primary culture of midbrain dopaminergic neurons. Results: There only existed the shorter GDNF transcript of 555 bp in the Han nationality. The secretory expression product of the shorter transcript in insect cells promoted the survival and differentiation of dopaminergic neurons. Conclusion: It is found that there is a 78 bp deletion in the Han nationality GDNF transcript compared with the reported 633 bp GDNF transcript. The 78 bp deletion does not affect the secretory expression and biological activity of GDNF mature protein.

关 键 词：gial cell line-derived neurotrophic factor gene cloning baculovirus expression system dopaminergic neuron 

分 类 号：R346[医药卫生—基础医学]